Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
A High-Density Map for Navigating the Human Polycomb Complexome.
A reference map of the human binary protein interactome.
The FBXL family of F-box proteins: variations on a theme.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
p53/FBXL20 axis negatively regulates the protein stability of PR55α, a regulatory subunit of PP2A Ser/Thr phosphatase.
Multimodal cell maps as a foundation for structural and functional genomics.
AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex
NEDD8:AcM-UBE2M binds CRL1 E3 ubiquitin ligase complex
CAND1 binds cytosolic CRL E3 ubiquitin ligases
COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
MyrG-DCUN1D3 binds CRL1 E3 ubiquitin ligase complex
Transfer of Ub from E2 to substrate and release of E2
Release of E3 from polyubiquitinated substrate
Polyubiquitination of substrate
Falcon deep research report for human FBXL20
-
FBXL20 is the substrate-recognition subunit of an SCF (CUL1-SKP1-FBXL20) E3 ligase that promotes ubiquitination and proteasomal degradation of VPS34/PIK3C3, binding VPS34 through its C-terminal LRR region (VPS34 C2 domain).
"FBXL20 acts as the substrate-recognition component of an **SCF (SKP1-CUL1-FBXL20)** E3 ubiquitin ligase that promotes **ubiquitination and proteasomal degradation** of **VPS34 (PIK3C3)**, the catalytic subunit of class III PI3K complexes."
-
DNA damage drives a p53 to FBXL20 to VPS34 checkpoint in which CDK-dependent VPS34 Thr159 phosphorylation creates a phosphodegron required for FBXL20-mediated ubiquitination.
"phosphorylation at **T159** is required for efficient FBXL20-mediated ubiquitination and degradation. Specifically, VPS34 **T159A** (nonphosphorylatable) is largely resistant, whereas **T159E** (phosphomimetic) remains sensitive"
-
By lowering VPS34 and PtdIns3P output, FBXL20 acts as a negative regulator of autophagy under genotoxic stress and slows endosomal degradation of receptors such as EGFR.
"FBXL20 knockdown increases VPS34 and increases an autophagy marker readout (LC3-II ratio), whereas FBXL20 overexpression lowers VPS34; collectively this supports FBXL20 as a **negative regulator of autophagy** under genotoxic stress"
-
FBXL20 contains a CAAX motif that is isoprenylated, directing it to membranes, and this localization is required for ubiquitylation-dependent degradation of RIM1.
"FBXL20 contains a **CAAX motif** that is **isoprenylated**, directing FBXL20 to **membranes**; this localization is required for ubiquitylation-dependent degradation of **RIM1** (RAB3-interacting molecule 1)"
Interaction of E3 with substrate and E2-Ub complex