PET191 curation notes

IBA / PAINT

• The pinned GOA snapshot has two GO_REF:0000033 rows, both with
  PANTHER:PTN002007797 in WITH/FROM.
• Current local PAINT for PTHR28627 still places those same IBD assertions on
  PTN002007797: GO:0005739 mitochondrion, seeded by yeast Pet191 and human
  COA5, and GO:0033617 mitochondrial respiratory chain complex IV assembly,
  seeded by yeast Pet191 itself.
• The broad mitochondrion IBA is a valid non-core localization; the more
  specific mitochondrial intermembrane space and inner-membrane association rows
  carry the precise sites.
• The complex IV assembly IBA is a core no-failure transfer. The target's own
  SGD:S000003795 source is expected because direct yeast Pet191 evidence was
  used to place the ancestral PAINT assertion.

Cached Literature

• PMID:8381337 is the founding genetic paper: PET191 is required to assemble
  active cytochrome c oxidase, but its product is not a subunit of the final
  enzyme.
• PMID:18503002 establishes Pet191 as a twin-CX9C complex IV assembly factor
  whose motif cysteines support function and disulfide-linked incorporation into
  a large inner-membrane-associated oligomeric complex. Its abstract also
  reported Mia40-independent import, but that import model is now disputed:
  PMID:22984289
• PMID:22984289 supports the mitochondrial intermembrane-space localization by
  Bax-release IMS proteomics and Pet191-specific protease protection.
• PMID:16823961 and PMID:24769239 are high-throughput mitochondrial proteome
  rows and do not add Pet191-specific mechanistic evidence in the cached text.

2024-2026 Literature Check

• The only recent PubMed hit for PET191/COA5 in the cytochrome c oxidase
  literature is the 2025 human COA5 complexome study, PMID:39779219. It supports
  an early complex IV assembly role for human COA5, especially around MTCO2
  biogenesis and incorporation: PMID:39779219. This is consistent with, rather than corrective of, the yeast PAINT
  transfer.