Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for PSEPK LeuD
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Assigns Q88LE7 as the small subunit of EC 4.2.1.33.
"Full=3-isopropylmalate dehydratase small subunit"
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Identifies the functional enzyme as a LeuC-LeuD heterodimer.
"Heterodimer of LeuC and LeuD."
QuickGO annotation snapshot for PSEPK LeuD
OpenScientist literature and database synthesis for PSEPK LeuD
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Identifies Q88LE7 as the non-catalytic small subunit that completes the LeuC-LeuD isopropylmalate dehydratase active site.
"*leuD* (Q88LE7, locus PP_1986) encodes the **small subunit of isopropylmalate isomerase"
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No direct KT2440 LeuD enzymology was found; kinetic, structural, and substrate-specificity details are transferred from orthologous enzymes.
"Kinetic parameters (Km, kcat), the exact stereochemistry, and the physiological reversibility have been measured in *Salmonella*/*E. coli*, not in *P. putida* itself;"