Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A map of the interactome network of the metazoan C. elegans.
A conserved role of Caenorhabditis elegans CDC-48 in ER-associated protein degradation.
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CDC-48.1 and CDC-48.2 interact with UFD-1/NPL-4 to form the conserved CDC-48(UFD-1/NPL-4) complex
"both p97 homologs interact with UFD-1/NPL-4 in a similar CDC-48(UFD-1/NPL-4) complex"
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RNAi depletion induces ER stress and sensitivity to unfolded protein accumulation
"RNAi mediated depletion of the corresponding genes induces ER stress resulting in hypersensitivity to conditions which induce increased levels of unfolded proteins in the ER lumen"
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Demonstrates evolutionarily conserved retro-translocation machinery at the ER
"these data suggest an evolutionarily conserved retro-translocation machinery at the endoplasmic reticulum"
Cell cycle progression requires the CDC-48UFD-1/NPL-4 complex for efficient DNA replication.
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CDC-48(UFD-1/NPL-4) complex is required for S phase progression
"Our analysis of the CDC-48(UFD-1/NPL-4) complex identified a general role in S phase progression of mitotic cells essential for embryonic cell division and germline development of adult worms"
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Developmental defects result from DNA replication checkpoint activation
"These developmental defects result from activation of the DNA replication checkpoint caused by replication stress"
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CDC-48 depletion causes reduced DNA content and decreased DNA synthesis
"DNA content is strongly reduced in worms depleted for CDC-48, UFD-1, and NPL-4. In addition, these worms show decreased DNA synthesis"
p97 Homologs from Caenorhabditis elegans, CDC-48.1 and CDC-48.2, suppress the aggregate formation of huntingtin exon1 containing expanded polyQ repeat.
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CDC-48.1 and CDC-48.2 directly bind huntingtin exon1 fragment
"CDC-48.1 and CDC-48.2 bound the Htt exon1 fragment directly"
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Suppress SDS-insoluble aggregate formation independently of nucleotides
"suppressed the formation of SDS-insoluble aggregates of Htt fragments containing 53 glutamine residues (HttQ53) independently of nucleotides"
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Modulate oligomeric states during aggregate formation suggesting chaperone function
"CDC-48.1 and CDC-48.2 also modulated the oligomeric states of HttQ53 during the aggregate formation"
An Afg2/Spaf-related Cdc48-like AAA ATPase regulates the stability and activity of the C. elegans Aurora B kinase AIR-2.
Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.
Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis.
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All six UBXN proteins (UBXN-1 to UBXN-6) directly interact with CDC-48.1
"All six UBXN proteins directly interacted with CDC-48.1 and CDC-48.2"
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UBXN-1, UBXN-2, UBXN-3 colocalize with CDC-48 in spermatocytes
"UBXN-1, UBXN-2 and UBXN-3 colocalized with CDC-48 in spermatocytes but not mature sperm"
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UBX cofactors control spermatogenesis via TRA-1A degradation
"these results suggest that UBXN-1, UBXN-2 and UBXN-3 are redundant cofactors for CDC-48/p97 and control spermatogenesis via the degradation of TRA-1A"
Positive cooperativity of the p97 AAA ATPase is critical for essential functions.
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N-terminal AAA domain has low ATPase activity
"The ATPase activity of the N-terminal AAA domain was very low at physiological temperature"
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C-terminal AAA domain shows high ATPase activity with positive cooperativity
"the C-terminal AAA domain showed high ATPase activity in a coordinated fashion with positive cooperativity"
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Positive cooperativity is critical for essential functions
"the positive cooperativity is critical for the essential functions of p97"
EGF signalling activates the ubiquitin proteasome system to modulate C. elegans lifespan.
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EGF signaling upregulates UPS genes including CDC-48 pathway components
"EGF signalling upregulates the expression of genes involved in the ubiquitin proteasome system (UPS)"
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UFD complex required for increased UPS activity in adults
"SKR-5 and the E3/E4 ligases that comprise the ubiquitin fusion degradation (UFD) complex are required for the increase in UPS activity observed in adults"
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UPS activity regulates protein homeostasis and lifespan
"EGF signalling alters protein homoeostasis in adults by increasing UPS activity and polyubiquitination, while decreasing protein aggregation"
CDC-48/p97 is required for proper meiotic chromosome segregation via controlling AIR-2/Aurora B kinase localization in Caenorhabditis elegans.
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CDC-48s required for proper chromosome segregation during meiosis
"CDC-48s are required for proper chromosome segregation during meiosis in C. elegans"
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Controls restricted localization of AIR-2 to cohesion sites of homologous chromatids
"CDC-48s control the restricted localization of AIR-2 to the cohesion sites of homologous chromatids in meiosis I"
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Depletion causes expansion of AIR-2 signals over entire length of meiotic chromosomes
"depletion of CDC-48s resulted in a significant expansion of signals for AIR-2 and phosphorylated histone H3 over the entire length of meiotic chromosomes"
The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A.
High-speed atomic force microscopic observation of ATP-dependent rotation of the AAA+ chaperone p97.
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CDC-48.1 forms hexameric ring
"p97 (also called VCP and CDC-48) is an AAA+ chaperone, which consists of a substrate/cofactor-binding N domain and two ATPase domains (D1 and D2), and forms a homo-hexameric ring"
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ATP binding induces rotation of N-D1 ring relative to D2 ring
"In the presence of ATP, the N-D1 ring repeatedly rotates ~23 ± 8° clockwise and resets relative to the D2 ring"
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Rotation induced by ATP binding to D2 domain
"Mutational analysis reveals that this rotation is induced by ATP binding to the D2 domain"
Characterization of C-terminal adaptors, UFD-2 and UFD-3, of CDC-48 on the polyglutamine aggregation in C. elegans.
Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression.
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UBXN-3/FAF1 binds CDT-1 and ubiquitylated proteins to promote CDC-48-dependent turnover
"UBXN-3/FAF1 binds to the licensing factor CDT-1 and additional ubiquitylated proteins, thus promoting CDC-48/p97-dependent turnover and disassembly of DNA replication factor complexes"
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CDC-48 and UBXN-3 associate with chromatin in nuclei
"cellular fractionation of C. elegans embryonic lysates confirmed high abundance of UBXN-3, CDC-48 and CDT-1 in purified nuclei"
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Regulates disassembly of DNA replication factor complexes
"promoting CDC-48/p97-dependent turnover and disassembly of DNA replication factor complexes"
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Controls progression of DNA replication fork
"progression of the DNA replication fork is coordinated by UBXN-3/FAF1"
Deep research report on cdc-48.1