Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa.
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ctpH mutants lose Pi taxis at high Pi concentrations; ctpH ctpL double mutants lose it at all concentrations.
"The ctpH ctpL double mutant was defective in P(i) taxis at any concentration ranging from 0.01 to 10 mM."
Two different mechanisms mediate chemotaxis to inorganic phosphate in Pseudomonas aeruginosa.
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The CtpH ligand binding domain binds Pi directly (ITC, KD 22 uM).
"Data analysis revealed that the binding of CtpH-LBD with Pi has a KD of 22 μM (Table 1)."