Differential regulation of the Chlamydomonas Nar1 gene family by carbon and nitrogen.
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NAR1.2 (LCIA) gives electrophysiological responses to both HCO3- and NO2- when expressed in Xenopus oocytes
"The electrophysiological response to HCO3- and NO2- provides evidence that NAR1.2 is involved in both HCO3- and NO2- transport."
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NAR1.2 has a predicted chloroplast transit peptide
"NAR1.1, NAR1.2, and NAR1.5 contain putative chloroplast transit peptides"
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Nar1.2 is carbon-regulated, independently of the nitrate regulator Nit2
"One gene, Nar1.2, was strongly carbon-regulated independently of Nit2"
Introducing an algal carbon-concentrating mechanism into higher plants: location and incorporation of key components.
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LCIA-Venus is confined to the chloroplast envelope in Chlamydomonas
"LCIA: Venus was confined to the chloroplast envelope"
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LCIA-GFP localizes to the chloroplast envelope in tobacco
"Fluorescent signals for LCIA: GFP were in the chloroplast envelope"
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Mature LCIA (without transit peptide) increases H14CO3- uptake in Xenopus oocytes
"Oocytes transformed with mLCIA: GFP or HLA3: GFP accumulated 2.0‐ and 2.7‐fold more 14C than water‐injected controls, respectively"
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Expression of LCIA in Arabidopsis did not improve growth
"The addition of LCIA or HLA3 did not confer a growth advantage to Arabidopsis plants"
Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii.
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LCIA is enriched in chloroplast envelope fractions and is localized to the chloroplast envelope
"LCIA was highly enriched in the CE fraction, where CE protein CCP1 (30) was also enriched."
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LCIA insertion mutants have decreased Ci affinity, especially at alkaline pH
"Insertion mutants of HLA3 and/or LCIA showed decreased Ci affinities/accumulation, especially in alkaline conditions where HCO3 (-) is the predominant form of Ci."
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Co-overexpression of HLA3 and LCIA increases Ci affinity
"simultaneous overexpression of HLA3 with LCIA significantly increased Ci affinity/accumulation"
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LCIA and HLA3 each form protein complexes, independently of each other
"HLA3 and LCIA formed protein complexes independently"
Acclimation to very low CO2: contribution of limiting CO2 inducible proteins, LCIB and LCIA, to inorganic carbon uptake in Chlamydomonas reinhardtii.
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LCIA is the same protein as NAR1.2
"Limiting CO2 Inducible A (LCIA; also named NAR1.2)"
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LCIA is associated with HCO3- transport; LCIB with CO2 uptake
"LCIB appears to function in a CO2 uptake system, whereas LCIA appears to be associated with a HCO3(-) transport system."
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The LCIA-LCIB double mutant cannot survive very low CO2
"suggests that both LCIA and LCIB are critical for survival in very low CO2"
The Chlamydomonas reinhardtii chloroplast envelope protein LCIA transports bicarbonate in planta.
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LCIA complements E. coli lacking carbonic anhydrases and the Arabidopsis betaca5 mutant in ambient CO2, consistent with HCO3- uptake
"Expression of LCIA restored growth in both systems in ambient CO2 conditions, which strongly suggests that LCIA is facilitating HCO3- uptake in each system."
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Direct evidence that LCIA moves HCO3- across membranes
"To our knowledge, this is the first direct evidence that LCIA moves HCO3- across membranes in bacteria and plants."
Structure of Chlamydomonas reinhardtii LciA guided the engineering of FNT family proteins to gain bicarbonate transport activity.
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Cryo-EM structure of LciA
"Here we report the cryogenic electron microscopy structure of Chlamydomonas reinhardtii LciA."
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LciA is described as a chloroplast envelope bicarbonate channel of the FNT family
"Limited CO2-inducible A (LciA), a chloroplast envelope bicarbonate channel belonging to the formate/nitrite transporter (FNT) family"
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Bicarbonate selectivity is set by Lys220 and by Ala117 and Val267 in the selectivity filter
"We found that bicarbonate selectivity is governed by electrostatic coordination mediated by Lys220 and steric constraint imposed by Ala117 and Val267 within the selectivity filter."
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The bacterial nitrite channel NirC can be engineered to conduct bicarbonate, showing that bicarbonate selectivity is a derived property of specific FNT members
"we successfully engineered the bacterial FNT family nitrite channel NirC through site-directed mutagenesis to gain bicarbonate transport activity"
Knockdown of limiting-CO2-induced gene HLA3 decreases HCO3- transport and photosynthetic Ci affinity in Chlamydomonas reinhardtii.
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Combined HLA3 and (off-target) LCIA knockdown strongly decreases Ci uptake, supporting a role for LCIA in envelope HCO3- transport
"additional evidence supporting a role for LCIA in chloroplast envelope HCO(3)(-) transport"
Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii.
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LciA identified as a CCM1-regulated low-CO2-inducible gene, proposed to be involved in Ci transport
"Among low-CO2 inducible genes, two novel genes, LciA and LciB, were identified, which may be involved in inorganic carbon transport."
Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii.
The Integration of Algal Carbon Concentration Mechanism Components into Tobacco Chloroplasts Increases Photosynthetic Efficiency and Biomass.
The Chlamydomonas reinhardtii Nar1 gene encodes a chloroplast membrane protein involved in nitrite transport.
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods