Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Purification and preliminary characterization of brain aspartoacylase.
Towards a proteome-scale map of the human protein-protein interaction network.
Identification of the zinc binding ligands and the catalytic residue in human aspartoacylase, an enzyme involved in Canavan disease.
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Human active-site mutagenesis identifies zinc-binding residues and a catalytic role for Glu178.
"His21, Glu24 and His116 were identified here for the
first time as the residues involved in the zinc-binding process."
Structure of aspartoacylase, the brain enzyme impaired in Canavan disease.
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Crystal structures were determined for recombinant human and rat ASPA.
"We
present crystal structures of recombinant human and rat aspartoacylase refined
to 2.8- and 1.8-A resolution, respectively."
Examination of the mechanism of human brain aspartoacylase through the binding of an intermediate analogue.
Expression and localization of myosin-1d in the developing nervous system.
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Controlled binding experiments support ASPA association with the MYO1D tail; catalytic regulation was proposed, not demonstrated.
"we determined that Myo1d TH1 interacts with the C-terminus of aspartoacylase"
New T530C mutation in the aspartoacylase gene caused Canavan disease with no correlation between severity and N-acetylaspartate excretion.
A proteome-scale map of the human interactome network.
Widespread macromolecular interaction perturbations in human genetic disorders.
Clinically Distinct Phenotypes of Canavan Disease Correlate with Residual Aspartoacylase Enzyme Activity.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Cloning of the human aspartoacylase cDNA and a common missense mutation in Canavan disease.
ASPA deacetylates NAASP to acetate and L-aspartate
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The curated human ASPA reaction produces acetate and L-aspartate from NAA in the cytosol.
"ASPA catalyses the hydrolysis of N-acetylaspartic acid (NAA) to produce acetate (CH3COO-) and L-aspartate (L-Asp)."
Aspartoacylase is a regulated nuclear-cytoplasmic enzyme.
Bimodal occurrence of aspartoacylase in myelin and cytosol of brain.