Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
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Extracts of a PA14 strain overexpressing PA1107 contained 35 pmol c-di-GMP per mg wet cell weight (Table 1, read in PMC full text; detection limit 12).
"Analysis of the phenotypes of DGC and PDE mutants and overexpressing clones revealed that certain virulence-associated traits are controlled by multiple DGCs and PDEs through alterations in c-di-GMP levels."
Specific control of Pseudomonas aeruginosa surface-associated behaviors by two c-di-GMP diguanylate cyclases.
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Plasmid RoeA raises c-di-GMP 22-fold (LC-MS), and the GGAAF mutant does not.
"expressing PA1107 on a plasmid significantly stimulated c-di-GMP levels versus those of the ΔPA1107 strain carrying the vector control (22-fold, P < 0.05). In contrast, expressing the PA1107-AA mutant variant did not result in a significant change in c-di-GMP levels"
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A purified PleD-RoeA GGDEF chimera has in vitro DGC activity; the catalytic mutant chimera does not.
"Both of these chimeras could be purified, but only the construct with the WT GGEEF domain demonstrated in vitro DGC activity (Fig. 2H)."
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The deletion reduces biofilm formation in PA14.
"A deletion mutation of the PA1107 ORF was constructed (∆PA1107), and a biofilm formation defect was observed at 24 h in the microtiter dish assay (Fig. 1A)."
Phenotypic and integrated analysis of a comprehensive Pseudomonas aeruginosa PAO1 library of mutants lacking cyclic-di-GMP-related genes.
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In PAO1 the roeA deletion increased flow-cell biomass, the opposite of the PA14 microtiter result.
"Only four mutants exhibited elevated biomass measurements, namely PA0847 (Zhang et al., 2019), PA1107 (roeA; Cole and Lee, 2016), PA4367 and PA3825"
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt