Q8MST5 is the selected 457-residue betaTub97EF sequence with tubulin GTPase and C-terminal tubulin domains. PMID:29084803(https://pubmed.ncbi.nlm.nih.gov/29084803/), DOI https://doi.org/10.1242/dev.156109, directly establishes target-dependent microtubule stabilization in hemocytes and gut and increased expression at 14°C. It supports the broad structural/process predictions independently of electronic annotations. The accessible standard cache is abstract-only, so detailed isoform-specific kinetics and a dedicated mitotic requirement are not claimed.
PMID:9428769(https://pubmed.ncbi.nlm.nih.gov/9428769/) and PMID:9628483(https://pubmed.ncbi.nlm.nih.gov/9628483/) provide experimental structural grounding for conserved tubulin GTP binding and hydrolysis. This is justified transfer of core tubulin chemistry; alpha-subunit nonexchangeable GTP and beta-subunit exchangeable GTP are not conflated. Fifteen GOA rows are reviewed, and the ProtNLM process is LSP relative to supported microtubule cytoskeleton organization.
The selected frozen UniProt record is retained in betaTub97EF-uniprot-source.json, with the complete original prediction metadata in betaTub97EF-predictions-source.json. It maps the exact accession to this FlyBase gene; current sequence identity does not establish the historical predictor input. Gene-level experiments support conserved functions unless a relevant isoform difference is established. Falcon research was requested with perplexity-lite fallback alongside publication caching. Primary publications and sequence observations, rather than AI summaries or ARBA assertions, support the curated conclusions.
The publisher full text was inspected, and selected primary excerpts record spindle staining and isoform caveats. Both exon-4B and exon-4C forms partially rescue betaTub56D loss; the main text states that “UASt-βTub97EF(4B) and UASt-βTub97EF(4C) provided only limited rescue.” The specific stability experiments use 4B, and cold induction differs between 4B and 4C. Sequence comparison establishes Q8MST5 equals RefSeq variant B, but does not equate that transcript label with exon4B. Main biological text therefore separates the major4B cold response from the selected sequence’s generic tubulin role. The mitotic IBA is retained as noncore based on direct spindle localization. The standard cache remains abstract-only; full publisher text and the accessible supplement were inspected through web access.
The completed Falcon report was read in full after the wrapper timeout; the original child returned a complete report. Its primary-paper synthesis agrees with the independently inspected publisher text: both 4B and 4C forms support a broad microtubule role, whereas the detailed stabilization experiments and cold induction concern the predominant 4B form. The report does not map Q8MST5 to the paper’s exon names, so that exact correspondence remains explicitly unresolved. Generic tubulin GTP chemistry is retained as supported family inference; a structural role does not mean the beta-tubulin subunit lacks GTPase activity. Proposed upstream temperature-regulatory pathways are not assigned from a different gene’s enhancer experiments. No additional annotation change is required from the provider report.