Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Expression cloning of beta 1,4 N-acetylgalactosaminyltransferase cDNAs that determine the expression of GM2 and GD2 gangliosides.
Analysis of melanoma cells stably transfected with beta 1,4GalNAc transferase (GM2/GD2 synthase) cDNA: relative glycosyltransferase levels play a dominant role in determining ganglioside expression.
Substrate specificity of beta 1,4-N-acetylgalactosaminyltransferase in vitro and in cDNA-transfected cells. GM2/GD2 synthase efficiently generates asialo-GM2 in certain cells.
Genomic organization and chromosomal assignment of the human beta1, 4-N-acetylgalactosaminyltransferase gene. Identification of multiple transcription units.
B4GALNT1 dimer transfers GalNAc to gangliosides
Disulfide bonds of GM2 synthase homodimers. Antiparallel orientation of the catalytic domains.
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The study assigns C80/C82-to-C412/C529 disulfides between subunits and attributes homodimer formation to those bonds.
"the intersubunit disulfides formed by both
Cys(80) and Cys(82) with Cys(412) and Cys(529) are responsible for formation of
the homodimer."
Alteration of ganglioside biosynthesis responsible for complex hereditary spastic paraplegia.
Conformational dynamics and membrane insertion mechanism of B4GALNT1 in ganglioside synthesis.
Expression cloning of beta 1,4 N-acetylgalactosaminyltransferase cDNAs that determine the expression of GM2 and GD2 gangliosides.