Gene Ontology annotation through association of InterPro records with GO terms
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InterPro domain-based evidence supports LFY as a DNA-binding transcription-factor family member but should be refined to more specific cis-regulatory DNA-binding and transcriptional activation terms.
"DR InterPro; IPR002910; FLO_LFY."
Annotation inferences using phylogenetic trees
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Phylogenetic propagation is consistent with the conserved LFY/FLO family assignment for Arabidopsis LFY.
"Q00958,Protein LEAFY,protein,3702,Arabidopsis thaliana,Arabidopsis thaliana (Mouse-ear cress),LFY,420,PTHR36079:SF1,PROTEIN LEAFY,True"
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
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ARBA inferred broad meristem identity maintenance for LFY, but the curated Arabidopsis evidence supports floral meristem determinacy and flower development more specifically.
"ARBA00091440,arba,GO:0010074,maintenance of meristem identity"
Transcriptional activation of APETALA1 by LEAFY.
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LFY directly induces early AP1 expression during floral fate acquisition.
"Use of steroid-inducible activation of LFY demonstrated that early expression of AP1 is a result of transcriptional induction by LFY."
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Direct AP1 induction by LFY does not require new protein synthesis and is developmentally restricted.
"This AP1 induction is independent of protein synthesis and occurs specifically in the tissues and at the developmental stage in which floral fate is assumed."
Quantitative trait loci for floral morphology in Arabidopsis thaliana.
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The cached abstract describes floral morphology QTL mapping but does not verify an LFY-specific flower development annotation.
"We propose several candidate genes that may underlie these QTL on the basis of positional information and functional arguments."
LEAFY, a Homeotic Gene That Regulates Inflorescence Development in Arabidopsis.
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LFY is required for development of flower-bearing metamers.
"These data suggest that the LFY gene is required for the development of type 3 metamers and that the transition from type 2 to type 3 metamers is a developmental step distinct from that between vegetative and reproductive growth"
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LFY acts upstream of floral organ identity genes.
"Results from double mutant analysis, showing that lfy-1 is epistatic to the floral organ homeotic gene ap2-6, are consistent with the hypothesis that a functional LFY gene is necessary for the expression of downstream genes controlling floral organ identity."
An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development.
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UFO physically interacts with LFY and helps recruit UFO to the AP3 promoter.
"Here, we show that UFO physically interacts with LFY both in vitro and in vivo, and this interaction is necessary to recruit UFO to the AP3 promoter."
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LFY and UFO act in a transcriptional complex at AP3.
"Furthermore, a transcriptional repressor domain fused to UFO reduces endogenous LFY activity in plants, supporting the idea that UFO acts as part of a transcriptional complex at the AP3 promoter."
Regulatory networks that function to specify flower meristems require the function of homeobox genes PENNYWISE and POUND-FOOLISH in Arabidopsis.
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LFY participates in floral meristem specification.
"floral meristems are specified on the periphery of the inflorescence meristem by the combined activities of the FLOWERING LOCUS T (FT)-FD complex and the flower meristem identity gene, LEAFY (LFY)."
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pny pnf lfy genetic interactions reduce floral specification.
"Genetic analysis shows that when mutations in pny and pnf genes are combined with lfy, a synergistic phenotype is displayed that significantly reduces floral specification and alters inflorescence patterning events."
Structural basis for LEAFY floral switch function and similarity with helix-turn-helix proteins.
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The original structural paper is not cached in this worktree; external abstract text supports LFY DNA-binding-domain structures bound to target promoter DNA.
"Here, we report crystal structures for the DNA-binding domain of Arabidopsis thaliana LFY bound to two target promoter elements."
Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and determinacy in a previously undefined pathway targeting APETALA1 and AGAMOUS-LIKE24.
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LFY and AP1 confer floral fate in inflorescence primordia.
"LEAFY (LFY) and APETALA1 (AP1) are key regulators of this transition and expression of these genes in primordia produced by the inflorescence meristem confers floral fate."
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lfy enhances floral meristem fate and determinacy defects in bop mutants.
"Loss-of-function bop1 bop2 mutants show subtle defects in inflorescence and floral architecture but in combination with lfy or ap1, synergistic defects in floral meristem fate and determinacy are revealed."
LATE MERISTEM IDENTITY2 acts together with LEAFY to activate APETALA1.
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LFY interacts physically with LMI2 while activating AP1.
"Like LFY, LMI2 activates AP1 directly; moreover, LMI2 and LFY interact physically."
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LFY is a central meristem identity regulator.
"LFY is considered to be a central meristem identity regulator because lfy null mutants cause a very dramatic delay in the meristem identity transition"
SWI2/SNF2 chromatin remodeling ATPases overcome polycomb repression and control floral organ identity with the LEAFY and SEPALLATA3 transcription factors.
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SYD and BRM physically interact with LFY and SEP3.
"SYD and BRM physically interact with two tissue- and stage-specific activators of AP3 and AG, the plant-specific transcription factor LFY, which is known to play a central role in class B and class C gene induction, and with the MADS box transcription factor SEP3"
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LFY is needed for SYD association at AP3 and AG regulatory loci.
"SYD and LFY association with the AP3 and AG regulatory loci peaks at the same time during flower patterning, and SYD binding to these loci is compromised in lfy and lfy sep3 mutants."
A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis.
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The study generated a high-throughput transcription factor-promoter interaction network; the cached main text does not discuss LFY individually.
"Here, we mapped a putative PXY-mediated transcriptional regulatory network comprising 690 transcription factor-promoter interactions in Arabidopsis"
Gibberellins promote flowering of arabidopsis by activating the LEAFY promoter.
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GA promotes LFY promoter induction during flowering.
"We have found that the failure of gibberellin-deficient ga1-3 mutants to flower in short days was paralleled by the absence of LEAFY promoter induction."
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Constitutive LFY can bypass the ga1-3 flowering failure in short days.
"A causal connection between these two events was confirmed by the ability of a constitutively expressed LEAFY transgene to restore flowering to ga1-3 mutants in short days."
UniProtKB record for Arabidopsis LFY (Q00958)
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UniProt curates LFY as a nuclear FLO/LFY-family protein.
"SIMILARITY: Belongs to the FLO/LFY family."
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UniProt records LFY homodimerization on DNA and interactions with SYD and BRM.
"SUBUNIT: Forms homodimer when associated to DNA. Interacts with SYD and BRM."
Falcon deep research synthesis for Arabidopsis LFY
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Falcon synthesis identifies LFY as a nuclear DNA-binding pioneer transcription factor.
"LFY (UniProt Q00958; *A. thaliana* At5g61850) is a nuclear, DNA-binding transcription factor and pioneer factor that drives floral fate primarily by activating AP1 and related floral regulators."
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LFY pioneer activity involves nucleosomal binding, H1 displacement and SWI/SNF recruitment.
"Its ability to engage nucleosome-occupied targets and locally unlock chromatin is supported by quantitative nucleosome overlap statistics (68% root; ~60% inflorescence), nucleosomal binding affinity (K_D ~5-10 nM at AP1), and ChIP-qPCR evidence for H1 displacement and SWI/SNF recruitment at AP1"
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UFO can redirect LFY to composite LUBS cis-elements.
"UFO redirects LFY to composite LUBS elements to activate specific organ identity genes (notably AP3)"
PANTHER family metadata for PTHR36079
PANTHER PTHR36079 member entries
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Arabidopsis LFY and rice FL are in the same Protein LEAFY PANTHER subfamily.
"Q00958,Protein LEAFY,protein,3702,Arabidopsis thaliana,Arabidopsis thaliana (Mouse-ear cress),LFY,420,PTHR36079:SF1,PROTEIN LEAFY,True"