Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Next-generation sequencing to generate interactome datasets.
Characterization of the cellular localization of C4orf34 as a novel endoplasmic reticulum resident protein.
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Tagged SMIM14 co-localises with calnexin and Sec61 and is an ER-resident single-pass membrane protein.
"Taken together, our results suggest that C4orf34 is a novel ER resident, type I transmembrane protein that might play a role in ER functions."
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ER retention maps to the transmembrane segment, not to a canonical retention motif.
"our results suggest that TMD of hC4orf34 might have an ER retention signal."
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SMIM14 transcript levels do not change during tunicamycin-induced ER stress.
"This result indicates that gene expression of hC4orf34 is not changed by ER stress (Fig. 4)."
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
SMIM14 - The Human Protein Atlas