Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Towards a proteome-scale map of the human protein-protein interaction network.
An empirical framework for binary interactome mapping.
Functional genetic screen of human diversity reveals that a methionine salvage enzyme regulates inflammatory cell death.
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APIP role in methionine salvage confirmed
"The role of APIP in methionine salvage was confirmed by growth assays with methionine-deficient media and quantitation of the methionine salvage substrate, 5'-methylthioadenosine"
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APIP variants affect susceptibility to both pyroptosis and apoptosis
"Reducing expression of APIP or exogenous addition of 5'-methylthioadenosine increased Salmonellae-induced cell death"
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APIP inhibits caspase-1 and caspase-9 mediated cell death
"Consistent with APIP originally being identified as an inhibitor of caspase-9-dependent apoptosis, the same allele was also associated with increased sensitivity to the chemotherapeutic agent carboplatin"
Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway.
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APIP is the human MtnB enzyme
"Using a bioinformatics approach, we propose that a protein called APIP could perform this role"
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APIP knockdown impairs growth in MTA medium
"We show that APIP depletion specifically impaired the capacity of cells to grow"
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APIP localizes to cytoplasm
"APIP was detected mainly in the cytoplasm of HeLa cells by immunofluorescence"
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Two isoforms exist (long and short)
"Two splice isoforms are described for APIP (APIP.long and APIP.short)"
Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.
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Crystal structure at 2.0 A (PDB 4M6R)
"The crystal structure was determined at 2.0-Å resolution, revealing an overall fold similar to members of the zinc-dependent class II aldolase family"
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Km = 9.32 uM, Vmax = 1.39 umol/min/mg for MtnB reaction
"Here we report the structural and enzymatic characterization of human APIP as an MtnB enzyme with a Km of 9.32 μM and a Vmax of 1.39 μmol min(-1) mg(-1)"
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Homotetramer with C4 symmetry
"APIP/MtnB exists as a tetramer in solution and exhibits an assembly with C4 symmetry in the crystal lattice"
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Glu139 is catalytic acid/base
"We propose an enzymatic reaction mechanism involving Glu139* as a catalytic acid/base"
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Anti-apoptotic activity is enzyme-independent
"APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide"
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Anti-pyroptotic activity is enzyme-dependent
"APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis"
A proteome-scale map of the human interactome network.
An inter-species protein-protein interaction network across vast evolutionary distance.
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Deep research report on APIP
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APIP competes with procaspase-9 for Apaf-1 binding
"APIP competes with procaspase-9 for Apaf-1 binding, inhibiting apoptosome-driven caspase-9 activation and offering cytoprotection in hypoxia/ischemia models"
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Enzymatic activity dispensable for anti-apoptotic effects
"APIP's enzymatic activity is dispensable for anti-apoptotic effects under hypoxia/etoposide, but required for suppression of caspase-1-dependent pyroptosis"