Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase.
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sgcA (sGC) is a class III cyclase homologous to mammalian soluble adenylyl cyclase, and its disruption reduces guanylyl cyclase activity more than 10-fold without affecting adenylyl cyclase activity.
">10-fold reduction in guanylyl cyclase activity"
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Physiological Mg2+-dependent sGC activity is stimulated by GTPgammaS and inhibited by Ca2+, indicating regulation distinct from the NO/heme metazoan soluble guanylyl cyclase.
"stimulated by GTPĪ³S and inhibited by Ca 2+ ions"
Chemotaxis: signalling modules join hands at front and tail.
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The cGMP-signalling cascade regulates myosin filament formation in the posterior of the cell, inhibiting lateral pseudopodia that could misdirect the cell.
"regulates myosin filament formation in the posterior of the cell"
Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis.
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In resting cells sGC-GFP is mainly cytosolic, and in a cAMP gradient it relocates to the anterior cell cortex where the membrane-associated enzyme becomes active.
"major fraction of the sGC-GFP fusion protein localizes to the cytosol"
Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells.
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The sGC protein is enriched at the leading edge and associates with actin filaments in pseudopodia, while its cGMP product drives cortical myosin in the rear; the two functions are separable.
"The sGC protein is enriched in extending pseudopodia at the leading edge of the cell during chemotaxis"
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sGC associates with actin filaments in pseudopodia.
"sGC protein associates with actin filaments in pseudopodia"
The role of cGMP and the rear of the cell in Dictyostelium chemotaxis and cell streaming.
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A quiescent, cGMP-dependent rear with efficient pseudopod retraction increases directional movement and stable cell streaming.
"quiescent rear of the cell increases the efficiency of directional movement"
Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum.
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A soluble guanylyl cyclase mediates chemotaxis by localizing to the leading edge (protein) and inducing rear myosin filaments (cGMP product).
"cyclic guanosine monophosphate product induces myosin filaments in the rear of the cell"
Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling.
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sGC and its cGMP product control directional switching in electrotaxis, with sGC localizing to the leading-edge pseudopod in an actin-dependent manner.
"components of the GCase- and PI3K-dependent signaling pathways localized at the leading edge of migrating cells during electrotaxis in an actin-dependent manner"
Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplification.
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sGC is an accessory signalling enzyme that is dispensable for basal Ras-driven chemotaxis but provides a memory of direction and improved orientation, amplifying sensitivity in shallow gradients.
"they provide a memory of direction and improved orientation of the cell"
Pumilio-dependent localization of mRNAs at the cell front coordinates multiple pathways required for chemotaxis.
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The four chemotaxis-pathway proteins (including sGC) and their mRNAs are preferentially enriched at the cell front during dynamic migration.
"proteins of these four chemotaxis pathways and actin are preferentially enriched at the cell front"
Intracellular Calcium Responses to External Calcium Stimuli in Dictyostelium.
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gca/sgc-null cells show an altered (slightly delayed) intracellular calcium response to external calcium, and cGMP is implicated in calcium homeostasis.
"gca/sgc-null cells showed a slightly delayed response"