Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Human gastrin-releasing peptide gene maps to chromosome band 18q21.
Posttranslational processing of endogenous and of baculovirus-expressed human gastrin-releasing peptide precursor.
-
Demonstrates that human prepro-GRP is cleaved at dibasic residues and alpha-amidated at its C-terminal methionine in neuroendocrine small-cell lung cancer cells to yield mature secreted GRP(1-27).
"In the small-cell lung cancer cell line, GRP1-27 was cleaved as expected from the endogenous prohormone at a pair of basic amino acids (29 and 30) and alpha-amidated at its C-terminal methionine"
A reference map of the human binary protein interactome.
Proglucagon gene expression is induced by gastrin-releasing peptide in a mouse enteroendocrine cell line.
-
Shows that gastrin-releasing peptide (GRP) stimulates proglucagon mRNA expression in STC-1 enteroendocrine cells via intracellular signaling cascades.
"We show here that gastrin-releasing peptide (GRP) induces proglucagon messenger RNA transcripts in STC-1 enteroendocrine cells."
Bombesin-like receptors bind bombesin homologues
-
Documents that gastrin-releasing peptide (GRP) and neuromedin B (NMB) are the two mammalian bombesin-like peptides that bind and activate the Gq/11-coupled bombesin receptors GRPR and NMBR.
"There are two homologues of bombesin-like peptides; Gastrin-releasing peptide (GRP) (Sausville EA et al, 1986) and Neuromedin-B (NMB) (Krane IM et al, 1988)."
Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
-
Documents that Gq/11-activating GPCRs activate phospholipase C (PLC) to hydrolyze PIP2 into DAG and IP3 and mobilize intracellular calcium.
"G alpha q protein (or Gq/11) consists of four family members (G-alpha 11, -alpha 14, -alpha 15 and -alpha q). It activates phospholipase C (PLC) (Dowal L et al, 2006)."
Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
The Ligand:GPCR:Gq complex dissociates