GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt subcellular-location mappings support nuclear envelope, nuclear pore, and nucleoplasm locations when consistent with experimental ELYS literature.

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

HPA immunofluorescence-derived locations are useful but must be interpreted against stronger ELYS NPC/chromatin/kinetochore evidence.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Automated nucleus/cytoplasm/nuclear-matrix calls are broad; nucleus is compatible, whereas cytoplasm is not a defining AHCTF1 location.

PMID:17098863

ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.

ELYS copurifies with Nup107-160, localizes to nuclear pores and kinetochores, and ELYS depletion disrupts nuclear pores and increases cytokinesis defects.

PMID:19056867

Large-scale proteomics and phosphoproteomics of urinary exosomes.

Urinary-exosome proteomics detected many proteins and does not establish AHCTF1 as a functional exosome component.

PMID:24315095

Integrated structural analysis of the human nuclear pore complex scaffold.

The human Nup107 scaffold forms reticulated NPC rings, supporting the structural context for AHCTF1 as a Nup107-160-associated NPC factor.

PMID:27016207

The Structure Inventory of the Nuclear Pore Complex.

NPCs are the principal gateway for nuclear-cytoplasmic exchange; ELYS is described as an optional/additional Y-complex-associated nucleoporin in some species.

PMID:27341616

Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation.

MEL-28/ELYS domains required for NPC, kinetochore, and chromatin localization are conserved, and the C-terminal region of human ELYS supports chromatin association.

Reactome:R-HSA-141409

Mad1 binds kinetochore

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-141422

MAD2 converted to an inhibitory state via interaction with Mad1

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-141431

MAD2 associates with the Mad1 kinetochore complex

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-141439

Release of activated MAD2 from kinetochores

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-1638803

Phosphorylation of cohesin by PLK1 at centromeres

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-1638821

PP2A-B56 dephosphorylates centromeric cohesin

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-2467809

ESPL1 (Separase) cleaves centromeric cohesin

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-2467811

Separation of sister chromatids

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-2468287

CDK1 phosphorylates CDCA5 (Sororin) at centromeres

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-2484822

Kinetochore assembly

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-375302

Kinetochore capture of astral microtubules

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-5666129

CDC42:GTP recruits DIAPH2-2 to kinetochores

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-5666160

AURKB phosphorylates DIAPH2-2 at kinetochores

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-5666169

Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

Reactome:R-HSA-9615901

AHCTF1 (ELYS) binds chromatin and Nup107-Nup160 complex

AHCTF1/ELYS binds chromatin and the Nup107-160 complex and acts as a seeding center for NPC assembly.

Reactome:R-HSA-9618378

POM121 binds the Nup107-Nup160 complex

Downstream POM121/Nup107-160 binding is part of NPC assembly but does not provide direct AHCTF1 cytosol-localization support.

Reactome:R-HSA-9634169

POM121 and NDC1 bind the Nup93 complex

Downstream POM121/NDC1/Nup93 recruitment is part of NPC assembly but does not provide direct AHCTF1 cytosol-localization support.

Reactome:R-HSA-9648114

EML4 recruits NUDC to mitotic spindle

Reactome mitotic pathway context does not by itself justify propagating a generic cytosol location to AHCTF1.

file:human/AHCTF1/AHCTF1-uniprot.txt

UniProtKB record for human AHCTF1/ELYS

Reviewed UniProt record summarizes AHCTF1 as a nuclear pore assembly factor that recruits Nup107-160 and localizes to NPCs, nuclear envelope, chromatin, and kinetochores.

file:human/AHCTF1/AHCTF1-deep-research-falcon.md

Falcon deep research review of human AHCTF1/ELYS

Falcon research summarizes AHCTF1/ELYS as a chromatin-binding nucleoporin that seeds post-mitotic NPC reassembly by recruiting the Nup107-160/Y-complex to decondensing chromosomes.
Falcon research highlights recent literature on ELYS nucleosome binding, VAPB interaction during mitosis, chromatin anchoring at NPCs, and cancer-relevant nuclear architecture contexts.