Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors.
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Purified recombinant kinesin-II behaves as a monodisperse heterotrimer of the KLP-11, KLP-20 and KAP-1 subunits (native mass ~287 kD, 1:1:1 stoichiometry), establishing klp-20 as a subunit of the heterotrimeric kinesin-II complex.
"the KLP-11, KAP-1, and KLP-20 subunits elute as a monodisperse heterotrimeric complex"
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Kinesin-II (containing klp-20) is an ATP-driven microtubule motor; in gliding assays it moves microtubules and uses Mg-ATP by Michaelis-Menten kinetics.
"indicating that Mg-ATP is the preferred substrate for kinesin-2 motors"
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Kinesin-II and OSM-3 are the two anterograde IFT motors that redundantly move IFT particles to build the sensory cilium foundation.
"two anterograde IFT motors called kinesin-II and OSM-3"
Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner.
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The KLP-11/KLP-20 heterodimer pairs an unprocessive with a processive motor domain; heterodimerization generates processivity.
"One motor domain is unprocessive as a homodimer"
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Heterodimerization of klp-20 with klp-11 is required to bind KAP-1, the in vivo link between the motor and its cargo.
"heterodimerization is necessary to bind KAP1, the in vivo link between motor and"