Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
YeastPathways to GO-CAM conversion
Inhibition of ergosterol biosynthesis in saccharomyces cerevisiae and Ustilago maydis by tridemorph, fenpropimorph and fenpropidin
The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy.
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Natter et al. used large-scale C-terminal GFP microscopy to localize yeast lipid-metabolism proteins, with most tagged lipid enzymes localizing to the ER.
"The majority of tagged enzymes localized to the endoplasmic reticulum (91)"
A systematic study of yeast sterol biosynthetic protein-protein interactions using the split-ubiquitin system.
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Mo and Bard reported a split-ubiquitin interaction between the sterol enzymes Erg24p and Erg2p.
"Erg24p and Erg2p, two enzymes that are sensitive to morpholine antifungals, appear to interact with one another"
Identification of essential amino acid residues in a sterol 8,7-isomerase from Zea mays reveals functional homology and diversity with the isomerases of animal and fungal origin.
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Rahier et al. used a yeast erg2 mutant lacking 8,7 sterol isomerase activity and corresponding S. cerevisiae isomerase mutants to analyze 8,7SI function.
"yeast erg2 mutant lacking 8,7SI activity"
Steroid-transforming enzymes in fungi.
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Kristan and Rizner reviewed fungal steroid-transforming enzymes, including ergosterol-pathway enzymes and their localization.
"The substrate specificities of these enzymes, their cellular localisation, their association with protein super-families, and their potential applications are discussed"
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
The social and structural architecture of the yeast protein interactome.
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Michaelis et al. generated a 2023 high-throughput affinity-enrichment mass spectrometry interactome of endogenously GFP-tagged yeast proteins.
"high-throughput method using highly reproducible affinity enrichment"
Yeast sterol C8-C7 isomerase: identification and characterization of a high-affinity binding site for enzyme inhibitors.
PANTHER PTHR10868 PAINT annotations
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PTN001280219 is the fungal PAINT node that carries the C-8 sterol isomerase and ergosterol-biosynthesis assertions for PTHR10868.
"PTHR10868 PTN001280219 GO:0000247 F IBD false CGD:CAL0000177093|SGD:S000004815 taxon:451864 20251127"
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PTN001328958 is the broader eukaryotic PAINT node that propagates only ER localization, not the animal Sigma-receptor-specific terms, to fungal ERG2 proteins.
"PTHR10868 PTN001328958 GO:0005783 C IBD false MGI:MGI:1195268|RGD:68364|SGD:S000004815|UniProtKB:Q99720 taxon:2759 20260528"
UniProtKB record for S. cerevisiae ERG2
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S. cerevisiae ERG2 is annotated as the ER-membrane C-8 sterol isomerase that catalyzes fecosterol to episterol during ergosterol biosynthesis.
"Reaction=fecosterol = episterol; Xref=Rhea:RHEA:33435"
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UniProt places S. cerevisiae Erg2p at the ER membrane.
"SUBCELLULAR LOCATION: Endoplasmic reticulum membrane"
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UniProt records a physical interaction between Erg2p and Erg24p.
"P32352; P32462: ERG24; NbExp=3"
GO-CAM index rows for S. cerevisiae ERG2
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The curated SGD Yeast ergosterol GO-CAM models ERG2 as a C-8 sterol isomerase in the ER.
"SGD:S000004815 ERG2 Scer 61f34dd300001044 Yeast ergosterol NCBITaxon:559292 gomodel:61f34dd300001044/6205c24300000137 GO:0000247 C-8 sterol isomerase activity GO:0006696 ergosterol biosynthetic process GO:0005783 endoplasmic reticulum"
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Imported YeastPathways models include the ERG2/RXN3O-203 ergosterol biosynthesis step but assign the activity to cytosol.
"SGD:S000004815 ERG2 Scer YeastPathways_ERGOSTEROL-SYN-PWY-1 superpathway of ergosterol biosynthesis I - imported from: Saccharomyces Genome Database NCBITaxon:559292 gomodel:RXN3O-203 GO:0003674 molecular_function GO:0006696 ergosterol biosynthetic process GO:0005829 cytosol"