Fatty acid biosynthesis in Pseudomonas aeruginosa: cloning and characterization of the fabAB operon encoding beta-hydroxyacyl-acyl carrier protein dehydratase (FabA) and beta-ketoacyl-acyl carrier protein synthase I (FabB).
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fabB encodes beta-ketoacyl-ACP synthase I, and fabA and fabB were cloned, sequenced and expressed.
"The Pseudomonas aeruginosa fabA and fabB genes, encoding beta-hydroxyacyl-acyl carrier protein dehydratase and beta-ketoacyl-acyl carrier protein synthase I, respectively, were cloned, sequenced, and expressed in Escherichia coli."
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Chromosomal fabB mutants are auxotrophic for unsaturated fatty acids.
"Chromosomal fabA and fabB mutants were isolated; the mutants were auxotrophic for unsaturated fatty acids."
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fabA and fabB are cotranscribed as an operon.
"Northern analysis demonstrated that fabA and fabB are cotranscribed and most probably form a fabAB operon."
Crystal structure of Pseudomonas aeruginosa FabB C161A, a template for structure-based design for new antibiotics.
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FabB elongates the cis-unsaturated intermediate cis-3-decenoyl-ACP through successive rounds of condensation.
"FabB is able to catalyse the elongation of cis-3-decenoyl-ACP up to three times, and synthesise cis-5-dodecenoyl-ACP, cis-7-tetradodecenoyl-ACP and cis-9-hexadodecenoyl-ACP."
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Loss of FabB makes cells auxotrophic for unsaturated fatty acids.
"Cells lacking FabB are auxotroph for unsaturated fatty acids, making FabB an essential gene for the bacteria."
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The final vaccenic acid condensation is performed by FabF, not FabB.
"In contrast, FabF but and not FabB was shown to be responsible for the condensation of cis-9-hexadodecenoyl-ACP to cis-11-octadodecenoyl-ACP, the last step for the synthesis of vaccenic acid."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods