Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of intracellular localizations of expressed fusion proteins in living cells
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
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Affinity-enrichment MS mapping of mitochondrial protein interactions identified a dynamic human coenzyme Q biosynthetic complex (complex Q / CoQ synthome) that includes COQ6, localizing it to the inner membrane as part of the CoQ biosynthetic complex.
"identify a dynamic human coenzyme Q biosynthetic complex that includes multiple MXPs"
Biochemistry of Mitochondrial Coenzyme Q Biosynthesis.
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Review establishing that the eukaryotic C5-hydroxylation of the CoQ head group is catalyzed by COQ6, a flavin (FAD)-dependent monooxygenase, and that the terminal-stage COQ proteins are peripherally associated with the matrix face of the mitochondrial inner membrane as complex Q.
"The C5-hydroxylation is catalyzed by COQ6 in eukaryotes"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis.
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In vitro reconstitution of the animal COQ metabolon demonstrated that COQ6 is a class A flavin-dependent monooxygenase that performs the C5 hydroxylation and, downstream of COQ4, also the C1 hydroxylation, using FAD and requiring the FDXR/FDX2 ferredoxin couple to supply electrons from NADPH.
"corroborating its dual functionality as both a C5 and C1 hydroxylase"
COQ7:COQ9 octamer hydroxylates DMQ10H2