Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Global regulatory logic for specification of an embryonic cell lineage.
-
erg is activated immediately downstream of the four double-negative-gate targets (alx1, ets1, tbr, tel) together with hex and tgif; erg and hex form an interlocking positive feedback loop and knockdown of either depresses the other.
"These genes are engaged in interlocking positive double feedback loops ( erg with hex ; and hex with tgif ). Interference with expression of any of these genes severely depresses transcript levels of the others ( Fig. S3 ). Thus they dynamically stabilize all aspects of the regulatory state downstream of themselves."
-
Erg is one of the drivers of the skeletogenic differentiation genes, serving as both an A and a B driver in the feed-forward subcircuits.
"The alx1 and erg genes play both roles, but the ets1 gene ( 9 ) is used in these gene batteries only as the A driver"
-
The erg-hex loop is a canonical dynamic state-lockdown circuit whose outputs also feed other GRN nodes.
"The tgif-hex and erg-hex loops in the skeletogenic micromere GRN are canonical examples. Outputs of these genes not only serve as drivers for one another but also feed multiple other nodes of the GRN, so that their activity ensures the persistence of surrounding activities."
Identification and developmental expression of the ets gene family in the sea urchin (Strongylocentrotus purpuratus).
-
Sp-Erg is one of 11 S. purpuratus ets genes and is expressed exclusively in mesenchyme cells and/or their progenitors during embryogenesis; it is not maternally expressed.
"five of the nine sea urchin ets genes expressed during embryogenesis are exclusively (Sp-Ets1/2, Sp-Erg, Sp-Ese) or additionally (Sp-Tel, Sp-Pea) expressed in mesenchyme cells and/or their progenitors."
Diversification of oral and aboral mesodermal regulatory states in pregastrular sea urchin embryos.
-
After skeletogenic mesenchyme ingression, erg (with hex and ets1/2) is activated specifically in the oral non-skeletogenic mesoderm, and its NSM expression expands when the aboral regulators gcm or gataE are knocked down.
"At this time three genes that are first expressed in the SM, erg, hex, and ets1/2 are activated specifically in the oral NSM (Fig. 1O–R)."
Sub-circuits of a gene regulatory network control a developmental epithelial-mesenchymal transition.
-
In Lytechinus variegatus, erg is one of the ten downstream PMC transcription factors required for EMT, and snail regulates erg while erg regulates hex.
"the 10 TFs downstream of those (tel, erg, hex, tgif, snail, twist, foxn2/3, dri, foxb, foxo) were also required for EMT."
A large-scale analysis of mRNAs expressed by primary mesenchyme cells of the sea urchin embryo.
-
PMC EST project (the UniProt RX reference); the erg cDNA was recovered from this PMC-enriched EST collection, which identified transcription factors among PMC-expressed mRNAs.
"The collection of newly identified proteins includes many candidate regulators of primary mesenchyme morphogenesis, including PMC-specific extracellular matrix proteins, cell surface proteins, spicule matrix proteins and transcription factors."
The sea urchin erg homolog defines a highly conserved erg-specific domain.
-
A Lytechinus variegatus genomic fragment of the erg homologue showed that, beyond the ETS domain, the protein shares an ERG/Fli-1-specific conserved region.
"the remainder of the sequence is only homologous with the human ERG and murine Fli-1 genes. This latter region, designated R, represents a highly conserved erg-specific domain."