Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
A mutation in the Arabidopsis HYL1 gene encoding a dsRNA binding protein affects responses to abscisic acid, auxin, and cytokinin.
The nuclear dsRNA binding protein HYL1 is required for microRNA accumulation and plant development, but not posttranscriptional transgene silencing.
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HYL1 is needed for miRNA accumulation and repression of target mRNAs.
"miRNA accumulation also depends on the activity of HYL1, a nuclear dsRNA binding
protein."
Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana.
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HYL1 exhibits dsRNA binding and preferential DCL1 association.
"DCL1, DCL3, HYL1 and the four
HYL1 homologs exhibit significant dsRNA-binding activity"
microRNA-directed phasing during trans-acting siRNA biogenesis in plants.
The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis.
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HYL1-DCL1 association improves cleavage efficiency and precision.
"These
results indicate that HYL1 has a function in assisting efficient and precise
cleavage of pri-miRNA through interaction with DCL1."
SERRATE is a novel nuclear regulator in primary microRNA processing in Arabidopsis.
The N-terminal double-stranded RNA binding domains of Arabidopsis HYPONASTIC LEAVES1 are sufficient for pre-microRNA processing.
Arabidopsis primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body.
Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants.
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DCL1 and HYL1 occupy nuclear dicing bodies and diffuse nucleoplasm.
"Using live-cell imaging, we show here that DCL1 and HYL1 colocalize
in discrete nuclear bodies in addition to being present in a low-level diffuse
nucleoplasmic distribution."
Structure of Arabidopsis HYPONASTIC LEAVES1 and its molecular implications for miRNA processing.
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HYL1 has a canonical RNA-binding dsRBD and a noncanonical dimerization-associated dsRBD.
"both HR1 and TR2 are canonical dsRBDs for dsRNA binding,
whereas HR2 of HYL1 is a non-canonical dsRBD harboring a putative dimerization
interface."
Evidence for network evolution in an Arabidopsis interactome map.
The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis.
Fast-forward genetics identifies plant CPL phosphatases as regulators of miRNA processing factor HYL1.
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CPL1 regulation involves SERRATE-mediated access to HYL1 and dephosphorylation.
"In the miRNA processing complex, SE functions as a scaffold to mediate CPL1
interaction with HYL1, which needs to be dephosphorylated for optimal activity."
The RNA-binding protein HOS5 and serine/arginine-rich proteins RS40 and RS41 participate in miRNA biogenesis in Arabidopsis.
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HOS5, RS40 and RS41 associate with HYL1/SE in nuclear speckles.
"all of these splicing-related factors
directly interact with both HYL1 and SE in nuclear splicing speckles."
The Protein Phosphatase 4 and SMEK1 Complex Dephosphorylates HYL1 to Promote miRNA Biogenesis by Antagonizing the MAPK Cascade in Arabidopsis.
SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production.
Extensive signal integration by the phytohormone protein network.
Epigenetic regulation of female germline development through ERECTA signaling pathway.
Falcon deep research for Arabidopsis DRB1/HYL1
UniProt record for Arabidopsis DRB1/HYL1
PANTHER PTHR11207 entries containing Arabidopsis DRB1
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1.
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Purified HYL1 increases DCL1 cleavage rate and accuracy without providing RNase III catalysis.
"We show that recombinant HYL1 and SE proteins accelerate the
rate of DCL1-mediated cleavage of pre- and pri-miR167b substrates and promote
accurate processing."
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Adding HYL1 to DCL1 increased precise pri-miR167b processing from 11% to 22% in the reconstituted assay.
"HYL1 increased the accuracy from 11% to 22%, whereas SE increased the accuracy to 41%."
Hyponastic Leaves 1 Interacts with RNA Pol II to Ensure Proper Transcription of MicroRNA Genes.
Parallel degradome-seq and DMS-MaPseq substantially revise the miRNA biogenesis atlas in Arabidopsis.
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HYL1 and SERRATE affect precursor structures and processing in substrate-dependent ways.
"DCL1 partners Serrate and HYL1 could
synergistically and independently impact processing patterns and in vivo RNA
secondary structures of pri-miRNAs."
Cached PTHR11207 PAINT ancestral assertions
DRB1 re-review evidence and access notes
OpenScientist adjudication of DRB1/HYL1 ribonuclease III activity
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The completed report concurs that HYL1 is a dsRNA-binding DCL1 cofactor; its architecture assessment used database records rather than a new domain scan.
"The domain-architecture calls rest on UniProt/Pfam/PANTHER records rather than a de novo HMMER/InterProScan run in this session."