Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Inducible overproduction of the Aspergillus nidulans pentafunctional AROM protein and the type-I and -II 3-dehydroquinases from Salmonella typhi and Mycobacterium tuberculosis.
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Purified M. tuberculosis AroQ is a heat-stable homododecameric type II 3-dehydroquinase with a very low Km, and complements an A. nidulans qutE mutant.
"shown to encode quinate-inducible 3-dehydroquinase activity that allows a qutE- mutant strain of A. nidulans to utilize quinate as sole carbon source."
The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction.
Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions.
The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases.
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M. tuberculosis aroQ encodes a 3-dehydroquinase related to fungal catabolic DHQases rather than to E. coli-type biosynthetic enzymes.
"This finding indicates a common ancestral origin for genes encoding the catabolic dehydroquinases of fungi and the biosynthetic dehydroquinases present in some prokaryotes."
Dehydratation of DHQ yields DHS
UniProtKB entry for Mycobacterium tuberculosis H37Rv aroQ (P9WPX7)
Reviewer notes for M. tuberculosis aroQ