Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
A role for the yeast SWI/SNF complex in DNA replication.
Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator.
Generation of superhelical torsion by ATP-dependent chromatin remodeling activities.
Transcription activator interactions with multiple SWI/SNF subunits.
Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription.
Targeting activity is required for SWI/SNF function in vivo and is accomplished through two partially redundant activator-interaction domains.
Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast.
Proteome survey reveals modularity of the yeast cell machinery.
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
Swi3p controls SWI/SNF assembly and ATP-dependent H2A-H2B displacement.
Architecture of the SWI/SNF-nucleosome complex.
Composition and Function of Mutant Swi/Snf Complexes.
The cellular economy of the Saccharomyces cerevisiae zinc proteome.
The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain.
Cryo-EM structure of SWI/SNF complex bound to a nucleosome.
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In the cryo-EM structure of S. cerevisiae SWI/SNF bound to a nucleosome, Swi1 resides in the Core module acting as a molecular hub; the extranucleosomal DNA important for nucleosome binding coincides with the trajectory of the Swi1 N-terminus, linking Swi1 to nucleosome/DNA engagement and activator-dependent recruitment.
"the importance of the extranucleosomal DNA in nucleosome binding to SWI/SNF in our experimental setup. Interestingly, this extranucleosomal DNA also coincides with the possible trajectories of the N-terminal regions of both Swi1 and Snf5"
Structure of the SWI/SNF complex bound to the nucleosome and insights into the functional modularity.
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Higher-resolution cryo-EM structure of the yeast SWI/SNF-nucleosome complex resolving the substrate recruitment module (SRM); the DNA-binding lobe (DBL) is built from Snf6, the N-termini of Swi1 and Snf5, and Snf12, which directly or indirectly bind nucleosomal DNA near the exit DNA. The Swi1 and Snf5 N-termini interact and may engage DNA through the ARID domain or via transcription coactivators, providing the structural basis for SWI/SNF targeting. A truncated Swi1 construct (residues 251-1336) was used for structure determination.
"DBL of SWI/SNF is composed of multiple elements, including Snf6, the N-termini of Swi1 and Snf5, and Snf12, which directly or indirectly bind to the DNA (Fig. 1c). Snf6 is crosslinked to the nucleosomal DNA9 and is in close proximity to the exit DNA. The N-termini of Swi1 and Snf5 interact with each other (Supplementary Fig. S4b), which may bind to the DNA of nucleosome either through the ARID domain directly, or indirectly through transcription coactivators10, providing the structural basis for their cooperation in SWI/SNF targeting."
Cell cycle control of the yeast HO gene, cis- and trans-acting regulators.
The social and structural architecture of the yeast protein interactome.
Five SWI genes are required for expression of the HO gene in yeast.
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast.
Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement.
TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9.
Subunits of the yeast SWI/SNF complex are members of the actin-related protein (ARP) family.
Falcon deep research report on SWI1 (Saccharomyces cerevisiae)
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Swi1 is a non-catalytic auxiliary recruitment/structural subunit of the yeast SWI/SNF ATP-dependent chromatin-remodeling complex; the ATP hydrolysis and mechanical remodeling are carried out by the Snf2/Swi2 ATPase, not Swi1.
"Swi1 functions as an auxiliary recruitment/structural subunit"
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Swi1 is not an enzyme catalyzing a specific reaction; ATP hydrolysis and mechanical nucleosome remodeling are performed by the SWI/SNF ATPase Snf2/Swi2.
"Swi1 is not itself described as an enzyme catalyzing a specific reaction; the **ATP hydrolysis and mechanical remodeling** is carried by the SWI/SNF ATPase (Snf2/Swi2 in yeast nomenclature)"
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In the cryo-EM structure of yeast SWI/SNF on a mononucleosome, Swi1 contributes to the substrate recruitment module (SRM) and the DNA-binding lobe (DBL) near the exit DNA, helping bind nucleosomal DNA directly (via ARID) or indirectly (via coactivators).
"Swi1 (construct residues 251–1336)** contributes to the **substrate recruitment module (SRM)** and specifically to the **DNA-binding lobe (DBL)** located near the **exit DNA**; Swi1 is proposed to help bind nucleosomal DNA either directly (via ARID) or indirectly (via coactivators)"
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The N-termini of Swi1 and Snf5 interact, and Swi1 forms the SRM DNA-binding lobe together with Snf6, Snf12 and the Snf5 N-terminus.
"the **N-termini of Swi1 and Snf5 interact** and that Swi1 contributes to the SRM DNA-binding lobe together with **Snf6, Snf12, and Snf5 N-terminus**"
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The Swi1 ARID (AT-rich interaction) domain (~aa 405-506) has been experimentally mapped and binds DNA non-specifically, so it does not confer sequence-specific cis-regulatory DNA recognition.
"an ARID region has been experimentally mapped and reported to bind DNA **non-specifically**"
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Swi1 provides coactivator/activator interfaces: it binds transcriptional activation domains from Gcn4, VP16, Hap4 and (in 2024) Ino2, enabling activator-dependent recruitment of SWI/SNF to promoters; the ARID alone is not sufficient for activator binding.
"Swi1 binds activation domains from multiple activators (historic and newly tested), and in 2024 it was shown to bind the Ino2 activation domains relevant to phospholipid gene expression"
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Classic genetics place SWI1/SWI/SNF in promoter-specific transcriptional activation, e.g. SWI1 is required for expression of the a-specific gene STE6.
"SWI1 was reported to be required for expression of the a-specific gene **STE6**"
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Swi1 acts in the nucleus as part of the SWI/SNF complex associated with chromatin/nucleosomes, positioned in the recruitment portion of the complex adjacent to nucleosomal exit DNA.
"Swi1 acts in the **nucleus**, associated with the **SWI/SNF complex on chromatin/nucleosomes**"
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Reviews describe Swi1 as a central architectural subunit ('molecular nexus') of the yeast SWI/SNF complex, which itself comprises ~12 subunits and regulates expression of roughly 5% of yeast genes.
"centrally located within SWI/SNF architecture, functioning as a"