Gene Ontology annotation through association of InterPro records with GO terms

InterPro correctly identifies the Xin repeat domain (IPR012510) and the XIRP1/XIRP2 family (IPR030072), supporting actin binding and cell junction localization assignments.

Annotation inferences using phylogenetic trees

IBA annotations transferred from human XIRP2 (A4UGR9) and mouse Xirp2 (Q702N8) are well-supported by phylogenetic conservation of the Xin repeat domain architecture and consistent functional characterization across mammalian orthologs.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt subcellular location mapping correctly assigns cell junction and anchoring junction localization, consistent with XIRP2's known presence at intercalated discs and myotendinous junctions.

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

Ensembl Compara transfers from human XIRP2 (A4UGR9) and mouse Xirp2 (Q4U4S6) provide the most informative annotations, including Z disc localization, alpha-actinin binding, and regulation of actin filament organization.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined IEA methods assign the general actin binding term, which is subsumed by the more specific actin filament binding term from IBA/Ensembl Compara.

file:BOVIN/E1BL04/E1BL04-uniprot.txt

UniProt entry E1BL04

UniProt identifies E1BL04 as XIRP2 with 26 PROSITE Xin repeats and 18 Pfam Xin domains, belonging to the Xin family. The protein is 3561 amino acids and localizes to cell junctions.
Expression data from Bgee shows the gene is expressed in rectus femoris and 30 other cell types/tissues, consistent with striated muscle expression.