Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal.
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Original discovery of API5/AAC-11 as anti-apoptotic factor
"a cDNA whose expression supports cell viability after withdrawal of growth factors"
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Leucine zipper domain essential for protective function
"The protective action of AAC-11 was abolished by mutation of leucines to arginines within the leucine zipper domain"
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Strong species conservation
"The AAC-11 gene shows strong species conservation"
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Cells survive serum withdrawal for up to 12 weeks when expressing AAC-11
"BALB/c3T3 fibroblasts that were stably transfected with AAC-11 cDNA were viable in serum-free medium for up to 12 weeks"
AAC-11 overexpression induces invasion and protects cervical cancer cells from apoptosis.
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AAC-11 overexpression protects cervical cancer cells from apoptosis
"The effect of AAC-11 on cultured cervical cancer cells was associated with antiapoptotic process"
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Extended survival under serum-free conditions
"Approximately 50% of the AAC-11 transfected cells in serum-free medium died after 2 weeks"
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Also promotes invasion (secondary function)
"Invasion of matrigel and adhesion to laminin by AAC-11 transfected CUMC-6 cells were increased"
FIF [fibroblast growth factor-2 (FGF-2)-interacting-factor], a nuclear putatively antiapoptotic factor, interacts specifically with FGF-2.
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Identified FIF (API5) as nuclear 55 kDa protein
"we have isolated a gene encoding a nuclear protein of 55 kDa, FIF (FGF-2-interacting-factor)"
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Specific interaction with FGF-2 but not FGF-1, FGF-3, or FGF-6
"which interacts specifically with FGF-2 but not with FGF-1, FGF-3, or FGF-6"
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C-terminal part has antiapoptotic properties
"the COOH-terminal part, but not the full-length FIF protein, has previously been shown to exhibit antiapoptotic properties"
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Multiple isoforms with different localizations
"In contrast to the full-length FIF protein, several deletion constructs were able to transactivate a lac-Z reporter gene"
Large-scale identification of mammalian proteins localized to nuclear sub-compartments.
The antiapoptotic protein AAC-11 interacts with and regulates Acinus-mediated DNA fragmentation.
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AAC-11 binds Acinus and prevents DNA fragmentation
"AAC-11, a survival protein whose expression prevents apoptosis that occurs on deprivation of growth factors, physiologically binds to Acinus and prevents Acinus-mediated DNA fragmentation"
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Protects Acinus from caspase-3 cleavage
"AAC-11 was able to protect Acinus from caspase-3 cleavage in vivo and in vitro"
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Leucine zipper domain required for Acinus binding
"AAC-11 possesses a leucine-zipper domain that dictates, upon oligomerization, its interaction with Acinus"
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AAC-11 depletion sensitizes cells to chemotherapy
"AAC-11 depletion markedly increased cellular sensitivity to anticancer drugs"
Defining the membrane proteome of NK cells.
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
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API5 detected as mRNA-binding protein
"We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known"
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2.
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API5 directly binds caspase-2 CARD domain
"API5 protein directly binds to the caspase recruitment domain (CARD) of caspase-2"
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Prevents caspase-2 dimerization and activation
"impedes dimerization and activation of caspase-2"
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Key mechanism of anti-apoptotic function
"recombinant API5 directly inhibits full length but not processed caspase-2"
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
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API5-DDX39B interaction confirmed
"We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins"
Deep research summary for API5