Gene Ontology annotation through association of InterPro records with GO terms
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization.
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Speve was discovered as a beta-catenin-dependent zygotic even-skipped orthologue expressed early in all vegetal blastomeres and later shifting to veg1 derivatives.
"Speve, an evenskipped orthologue expressed very early in all vegetal blastomeres and then gradually shifting to veg(1) derivatives by the mesenchyme blastula stage"
A spatially dynamic cohort of regulatory genes in the endomesodermal gene network of the sea urchin embryo.
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The eve cis-regulatory module is activated by beta-catenin/Tcf and Blimp1, and repressed by Hox11/13b within the torus and by Tcf outside it.
"We verify the cis-regulatory inputs of even-skipped predicted by network analysis. These include activation by β-catenin/Tcf and Blimp1, repression within the torus by Hox11/13b, and repression outside the torus by Tcf in the absence of Wnt8 signal input."
The endoderm gene regulatory network in sea urchin embryos up to mid-blastula stage.
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Up to 18 h the only perturbation that changes eve expression is eve MASO itself, showing autorepression; eve MASO affects no other gene analyzed.
"The only perturbation resulting in a change of eve expression levels was injection of eve MASO itself."
A gene regulatory network controlling the embryonic specification of endoderm.
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Eve activates hox11/13b in veg1 descendants from 24 h, spatially activating the veg1 endoderm GRN that specifies hindgut fate.
"Hox11/13b expression is activated by Eve in descendants of veg1 cells at 24 h, because injection of eve MASO reduces hox11/13b expression only after 24 h (Fig. 3b)."
Specific functions of the Wnt signaling system in gene regulatory networks throughout the early sea urchin embryo.
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Eve MASO decreases wnt1, wnt4, wnt5 and wnt16; Eve represses animal-ectoderm genes in veg1 cells and activates wnt4, thereby driving veg1 ectoderm specification.
"Thus Eve plays a dual role in the specification of veg1 ectoderm: It directly represses genes of the animal ectoderm in these cells, and it activates the expression of wnt4 in veg1 endoderm and veg1 ectoderm, leading to the activation of genes of the veg1 ectodermal GRN."
Geometric control of ciliated band regulatory states in the sea urchin embryo.
microRNA-31 regulates skeletogenesis by direct suppression of Eve and Wnt1.
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eve is a direct target of miR-31; removing miR-31 suppression or overexpressing Eve causes PMC patterning and skeletogenic defects.
"Removal of miR-31 suppression of Eve and overexpression of Eve result in skeletogenic and PMC patterning defects."