2026-10-23: just deep-research-falcon ECOLI ccmE was attempted and failed with
Exception: Failed to authenticate; no other provider credentials were available.
This review is therefore based on the cached publications and the UniProt record.
Binds heme transiently and covalently as a membrane-anchored periplasmic chaperone and delivers it to the heme-ligation machinery. The E. coli evidence is distinguished from heterologous ortholog assays and engineered expression constructs.
Read the full 2023 E. coli structures (PMID:37626034), 2025 CcmB efflux work (PMID:40026579) and CcmCD crosslink mapping (PMID:40167305). The studies disagree about the heme conduit. ATP-dependent holo-CcmE release and covalent loading remain supported; heme contact, engineered overexpression, and proposed channel models are distinguished from purified vectorial transport. Broad intracellular heme handoff is not equivalent to membrane translocation. The 2025 review PMID:40460019 is abstract-only and is not used to settle this disagreement.
Specific reassessment: Binds heme transiently and covalently as a membrane-anchored periplasmic chaperone and delivers it to the heme-ligation machinery.
Default Falcon was genuinely attempted and failed with HTTP403 authentication; no provider report was created. Manual literature assessment is recorded here. Original seed rows (including inherited NEW), raw UniProt/GOA, and existing provider files are preserved against original-members-baseline.json. Focused validation and helper history are recorded in the original-panel audit ledger.
Current GO definitions were checked against the official QuickGO API. All original annotation payloads, including authored NEW rows, are preserved. Primary scope and construct limits remain explicit; no raw UniProt/GOA, provider report or publication cache was edited. Scoped comparative proof is recorded in the round-1 audit.
The historical NEW GO:0018063 duplicates current MODIFY replacements. It is retained by explicit parent instruction to preserve the frozen assertion; archival withdrawal would require a separate exact-payload preservation change and aggregate-checker reconciliation. This is annotation redundancy, not a disputed molecular role.
Narrowed the top-level finding statements for PMID:40167305 to the claims established by their existing quoted passages. Annotation actions, core functions, quotes and source records are unchanged. The original 52-file handoff remains preserved and verified separately; this edit has a supplemental history and manifest.
The redundant historical GO:0018063 NEW proposal is now retired in place using the schema's retired flag. Its row order, source payload, supporting quotations and historical action remain, with the exact prior row additionally recorded in analysis/module_audits/bacterial_cytochrome_c_maturation_system_i/retired-ccme-proposal.yaml. The true process remains asserted by the two GO:0017004 MODIFY replacements and by the core function; no REMOVE action is used for duplication. The core list omits the redundant GO:0017003 ancestor while retaining its distinct His130-adduct chemistry in prose and the accepted original annotation. GO:1903607 is retained: it is not in the current is_a/part_of ancestor closure of GO:0018063, so no unsupported hierarchy equivalence is asserted.
Standard record-section backfill derives the UniProt quote provenance; these metadata additions do not change the quoted source text or underlying functional assertions.