GTPBP6 (Putative GTP-binding protein 6, PGPL) — research notes

UniProt O43824. TRAFAC-class OBG-HflX-like GTPase. Pseudoautosomal gene (Xp/Yp telomere), escapes X-inactivation, Y homologue PMID:9466997.

Function — mitochondrial ribosome biogenesis & recycling

Despite being annotated by some IBA pipelines (from bacterial ObgE/CgtA ortholog P25519) as cytoplasmic ribosome-binding, the experimentally characterized human function is in MITOCHONDRIA:
- PMID:34135319
- PMID:34135319
- PMID:34135319
- PMID:34800366 mito proteome — GTPBP6 localizes to mitochondrion (HTP).

So: localization = mitochondrion (mitochondrial matrix/mitoribosome), NOT cytoplasm. MF = GTP binding + ribosomal large subunit binding (the mtLSU). BP = mitochondrial large ribosomal subunit assembly + (mitochondrial) ribosome recycling.

Decisions

• ribosome binding (IBA, from bacterial ortholog) — the real binding is to the mtLSU; MODIFY/keep general or KEEP_AS_NON_CORE; the specific ribosomal large subunit binding (IDA 34135319) is core.
• cytoplasm (IBA is_active_in, from bacterial ortholog P25519) — the human protein acts in the mitochondrion; this IBA mislocalizes; MARK_AS_OVER_ANNOTATED / flag. Mitochondrion (HTP 34800366) is correct.
• GTP binding (IEA InterPro, TAS 9466997) — ACCEPT (G-domain motifs; core MF support).
• ribosomal large subunit binding (IDA 34135319) — ACCEPT core (binds mtLSU).
• mitochondrial large ribosomal subunit assembly (IDA 34135319) — ACCEPT core BP.
• mitochondrion (HTP 34800366) — ACCEPT.