PANTHER family review PTHR24058: IBA propagation assessment for pom1
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Fission yeast Pom1p kinase activity is cell cycle regulated and essential for cellular symmetry during growth and division.
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Pom1 kinase activity is cell-cycle regulated and required for cellular symmetry; Pom1 needs its non-catalytic domain for cell-periphery localization and its kinase activity for cell-end localization.
"Pom1p is present in a large protein complex and requires its non-catalytic domain to localize to the cell periphery and its kinase activity to localize to cell ends."
Different mechanisms of cell polarisation in vegetative and shmooing growth in fission yeast.
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Pom1 is among the morphological factors concentrated at cell ends during vegetative growth and has a role early in the switch to shmooing growth.
"In vegetative growth these morphological factors are concentrated at cell ends, whereas during shmooing growth they are delocalised from the cell ends."
Mac1, a fission yeast transmembrane protein localizing to the poles and septum, is required for correct cell separation at high temperatures.
Role of Tea1p, Tea3p and Pom1p in the determination of cell ends in Schizosaccharomyces pombe.
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Pom1 contributes to cell-end determination; pom1 deletion (with tea1 or tea3) gives the most defective growth pattern and difficulty relocalizing actin to cell ends.
"has the greatest difficulty in relocalizing actin to the cell ends following actin depolymerization and generates the most defective growth pattern."
Pom1 DYRK regulates localization of the Rga4 GAP to ensure bipolar activation of Cdc42 in fission yeast.
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Pom1 physically interacts with and regulates the Cdc42 GAP Rga4 to ensure bipolar activation of Cdc42 and bipolar growth.
"Pom1 kinase physically interacts with Rga4, which has a GAP (GTPase- activating protein) domain for Rho-family GTPase."
A spatial gradient coordinates cell size and mitotic entry in fission yeast.
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Pom1 forms a polar gradient acting as a dose-dependent inhibitor of mitotic entry through the Cdr2 pathway.
"Pom1 forms a polar gradient extending from the cell ends towards the cell middle and acts as a dose-dependent inhibitor of mitotic entry, working through the Cdr2 pathway."
Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycle.
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A polar Pom1 gradient measures cell length and controls mitotic entry; Pom1 is a dose-dependent G2-M inhibitor that negatively regulates Cdr1/Cdr2.
"emanates from the ends of rod-shaped Schizosaccharomyces pombe cells, serves to measure cell length and control mitotic entry."
Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein.
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Pom1 is a negative regulator of the Pmk1 cell-integrity MAPK pathway, acting through Rga4 localization/phosphorylation (independent of Rga4's Rho2-GAP role).
"is also a negative regulator of the Pmk1 pathway, but this control is not dependent upon the Rga4 role as a Rho2-GAP."
Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation.
A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane.
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Tea4 recruits Pom1 to the cortex; Pom1 binds plasma-membrane lipids via a basic region and autophosphorylates to lower lipid affinity and release from the membrane, shaping the gradient.
"Pom1 autophosphorylates in this region to lower lipid affinity and promote membrane release."
Roles of the DYRK kinase Pom2 in cytokinesis, mitochondrial morphology, and sporulation in fission yeast.
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DYRK kinases including Pom1 autophosphorylate on tyrosine and phosphorylate exogenous substrates on serine/threonine (dual specificity).
"autophosphorylation on tyrosine and exogenous phosphorylation on serine/threonine residues."
Pom1 and cell size homeostasis in fission yeast.
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pom1-deleted cells still show wild-type size homeostasis, indicating Pom1 is not the unique/direct cell-size sensor even though it regulates the timing of mitotic entry.
"show wild-type size homeostasis both in size variability analyses and size homeostasis experiments."
Distinct levels in Pom1 gradients limit Cdr2 activity and localization to time and position division.
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Distinct Pom1 gradient thresholds limit Cdr2 activity/localization; Pom1 inhibits Cdr2 for mitotic commitment independently of regulating its localization.
"Pom1 inhibits Cdr2 for mitotic commitment independently of regulating its localization or cortical levels."
Dueling kinases regulate cell size at division through the SAD kinase Cdr2.
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Pom1 phosphorylates the Cdr2 C-terminal domain to prevent Cdr2 activation by the CaMKK Ssp1, opposing the Cdr2-Wee1 pathway.
"prevent activation of Cdr2 kinase activity by the CaMKK Ssp1."
Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
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Purified Pom1 directly phosphorylates multiple cell-polarity substrates in vitro, confirming them as direct Pom1 targets.
"Purified Pom1 phosphorylated these cell polarity targets in vitro, confirming that they are direct substrates of Pom1 kinase activity and likely contribute to regulation of polarized growth by Pom1."
Pom1 gradient buffering through intermolecular auto-phosphorylation.
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Pom1 autophosphorylates intermolecularly, conferring robustness to its gradient.
"auto-phosphorylates intermolecularly."
PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.
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Under glucose limitation Pom1 re-localizes to the cell sides (lateral cortex), where it strongly delays mitosis.
"cell sides upon environmental glucose limitation, where it strongly delays mitosis."
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
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Super-resolution mapping places Pom1 among signaling components of the contractile-ring intermediate layer.
"intermediate layer (80-160 nm) consists of a network of cytokinesis accessory proteins as well as multiple signaling components which influence cell division."
Mechanisms Connecting the Conserved Protein Kinases Ssp1, Kin1, and Pom1 in Fission Yeast Cell Polarity and Division.
Cell size-dependent regulation of Wee1 localization by Cdr2 cortical nodes.
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Pom1 contributes to size-dependent signaling by suppressing Wee1 node bursts in small cells (as a Cdr2 inhibitor).
"Size-dependent signaling was caused in part by the Cdr2 inhibitor Pom1, which suppressed Wee1 node bursts in small cells."
Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.
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The microtubule / Tea1-Tea4-Pom1 axis counteracts inappropriate Gef1 activity by regulating Rga4 localization, coordinating Cdc42 regulators for bipolar polarity.
"Tea1-Tea4-Pom1 axis counteract inappropriate Gef1 activity by regulating the localization of the Cdc42 GTPase-activating protein Rga4."
DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division.
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Pom1 kinase activity phosphorylates Cdc15 to drive it from the membrane and prevent septation at cell tips, ensuring medial division even without Mid1.
"kinase activity prevents septation at cell tips even if Mid1 is absent or mislocalized."
Multiple polarity kinases inhibit phase separation of F-BAR protein Cdc15 and antagonize cytokinetic ring assembly in fission yeast.
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Phosphorylation of Cdc15 by assorted polarity kinases (including Pom1) prevents Cdc15 condensation on the plasma membrane and antagonizes contractile-ring assembly.
"phosphorylation by assorted kinases prevents Cdc15 condensation on the PM and antagonizes CR assembly."
Polarity kinases that phosphorylate F-BAR protein Cdc15 have unique localization patterns during cytokinesis and contributions to preventing tip septation in Schizosaccharomyces pombe.
Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and cytokinesis.
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Pom1 provides positional information for both polarized growth and cytokinesis; mutants mostly fail to switch to bipolar growth and misplace septa.
"provides positional information for both polarized growth and cytokinesis."
Role of polo kinase and Mid1p in determining the site of cell division in fission yeast.