GTPBP3 review notes

Deep research

Automated deep research could not be generated for this review: just deep-research-falcon
failed (Edison API 402 Payment Required), the perplexity-lite fallback had no key, and
just deep-research-openai failed with 401. The review is built directly from the cached
primary literature listed below plus the UniProt record.

Literature used

• Villarroya et al. 2008 PMID:18852288
• Asano et al. 2018 PMID:29390138; substrates are 5,10-CH2-THF and taurine, reconstituted with the GTPBP3-MTO1 complex.
• Peng et al. 2021 PMID:33619562; also describes the cytoplasmic splice isoform (UniProt isoform 4) PMID:33619562.
• Kopajtich et al. 2014, COXPD23 disease-gene paper PMID:25434004.

Curation decisions

• All 26 protein binding IPI rows come from binary Y2H interactome screens with
  partners unrelated to mitochondrial tRNA modification; removed as uninformative. The
  meaningful interaction (MTO1) is captured by GO:7770010 GTPBP3-MTO1 complex.
• tRNA methylation (IBA) marked over-annotated: the MnmE/MnmG reaction adds a
  taurinomethyl (methylene-taurine) group at C5 of U34, not a methyl group.
• cytoplasm IBA marked over-annotated (bacterial MnmE compartment); the EXP/IEA
  cytoplasm rows are kept as non-core because they reflect the minor isoform 4.

Disease context (dismech)

dismech Combined_Oxidative_Phosphorylation_Defect_Type_23 (COXPD23) is the corresponding
disease entry; it was used only as a lead for literature.