Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
Autophagic cell death: analysis in Dictyostelium.
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GbfA is among the genes whose mutation was used to genetically dissect autophagic cell death, dissociating vacuolisation from cell death.
"These mutations allowed the genetic dissection of ACD features, dissociating in particular vacuolisation from cell death."
Identification of a target for CudA, the transcription factor which directs formation of the Dictyostelium tip organiser.
The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.
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EGCG treatment delays expression of late-aggregate/early-mound genes including gbfA.
"The delays in expression were also observed for tgrC and gbfA, genes expressed from late aggregate to early mound stage"
Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum.
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gbfA is expressed during the transition from aggregates to mounds.
"Two transcription factors involved in the transition from aggregates to mounds, gbfA and comH [29,30], also displayed temporally consistent expression between treatments"
Transcriptomic and metabolomic insights into light-mediated unicellular-to-multicellular transition in Dictyostelium discoideum.
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gbfA is a post-aggregation regulator whose transcript is downregulated in the dark during the unicellular phase.
"G-box binding factor GbfA and TgrC1 (lagC) have also reported to be involved in the post-aggregation stage [42], both of which were downregulated under the dark condition from transcriptomic analysis in the unicellular phase."
A GBF-binding site and a novel AT element define the minimal sequences sufficient to direct prespore-specific expression in Dictyostelium discoideum.
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In vitro-translated GBF binds all three SP60/cotC CA-rich elements (CAEs) in a sequence-specific manner.
"in vitro-transcribed and -translated GBF binds all three SP60/cotC CAEs in a sequence-specific manner"
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GBF has a specific role in prespore gene activation in addition to prestalk genes.
"it also has a specific role in prespore gene activation"
Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in Dictyostelium.
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GBF is a developmentally regulated transcription factor whose DNA affinity correlates with cAMP-inducibility of late gene promoters.
"whose affinity for a DNA sequence correlates with the ability of that sequence to confer inducibility to late gene promoters in response to high, continuous levels of extracellular cAMP"
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The GBF protein is highly basic and contains two putative zinc fingers.
"The predicted protein is highly basic and contains two putative zinc fingers."
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GBF disruption abolishes DNA-binding activity, arrests development at the loose aggregate stage, and eliminates late-gene induction.
"Disruption of the GBF gene by homologous recombination results in the loss of all GBF DNA-binding activity, developmental arrest at the loose aggregate stage, and the loss of late gene induction during development or in response to extracellular cAMP."
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GBF is a cAMP-responsive transcriptional activator essential for the switch between aggregation and cellular morphogenesis.
"GBF acts as an extracellular cAMP-responsive transcriptional activator regulating late gene expression and is an essential component of a developmental switch between aggregation and cellular morphogenesis"
lagC-null and gbf-null cells define key steps in the morphogenesis of Dictyostelium mounds.
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gbf-null cells fail to induce cell-type-specific genes of multicellular development.
"lagC-null and gbf-null cells fail to induce cell-type-specific genes ordinarily expressed during multicellular development."
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gbf-null mounds cannot establish a single dominant cAMP signaling-wave center.
"One reason for this was the inability of the mutant mounds to establish a single, dominant signaling-wave center."