Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription
Cell cycle control of the yeast HO gene cis and trans-acting regulators
Five SWI genes are required for expression of the HO gene in yeast
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex
A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast
Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement
Generation of superhelical torsion by ATP-dependent chromatin remodeling activities
The nuclear actin-related proteins Arp7 and Arp9 a dimeric module that cooperates with architectural proteins for chromatin remodeling
Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast
Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits
The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles
Proteome survey reveals modularity of the yeast cell machinery
Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae
Swi3p controls SWI/SNF assembly and ATP-dependent H2A-H2B displacement
Architecture of the SWI/SNF-nucleosome complex
The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation
Composition and Function of Mutant Swi/Snf Complexes
Cryo-EM structure of SWI/SNF complex bound to a nucleosome
The social and structural architecture of the yeast protein interactome
SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2
TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9
Subunits of the yeast SWI/SNF complex are members of the actin-related protein (ARP) family
Deep research report on SWI3
Falcon (Edison) deep research report on SWI3 (YJL176C, P32591)
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SWI3 (YJL176C) is a core, non-catalytic structural/regulatory subunit of the
SWI/SNF chromatin remodeling complex; it is not an enzyme.
"SWI3 is **not an enzyme**; it is a **core structural/regulatory subunit** within SWI/SNF."
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The Swi3 SWIRM domain directly binds DNA (including cruciform/nucleosomal DNA)
with apparent Kd ~72-105 nM in vitro, and contributes to SWI/SNF assembly and stability.
"and a KD of **~105.36 ± 7.65 nM** reported for binding to cruciform DNA in vitro."
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Cryo-EM resolves two copies of Swi3 (Swi3A/Swi3B) that, with Swi1, form the
structural backbone of the complex; Swi3 SWIRM domains contact Snf5 RPT regions.
"The model contains **two Swi3 instances (Swi3A and Swi3B)**."
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Swi3 is predominantly nuclear under normoxia but relocalizes to the cytosol under
hypoxia (>95% cytosolic), reversing on reoxygenation.
"Swi3 is predominantly **nuclear** under normoxia but undergoes regulated **cytosolic relocalization under hypoxia** (>95% cytosolic)"
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Beyond activation, Swi/Snf (and Swi3) contributes to context-specific gene
repression via transcriptional interference, shown by the swi3-E815X allele.
"A 2024 Molecular Cell study found that SWI/SNF remodeling regulates transcriptional interference and gene repression."
Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae
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The Swi3 paralog Swh3 (which partners with Sth1 in an essential RSC-related
complex) is functionally distinct from Swi3 and does not substitute for it,
aiding disambiguation of SWI3 from SWI3-like proteins.
"Swh3 and Swi3 are functionally"
Structure of the yeast Swi/Snf complex in a nucleosome free state
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Cryo-EM shows the conserved subunits Swi1 and Swi3 form the backbone of the
Swi/Snf complex, with a Swi3 dimer (coiled-coils, SANT, SWIRM domains)
enwrapping Swi1.
"Swi1 and Swi3 form the backbone of the complex and closely connect with surrounding subunits."
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The Snf5 RPT domains interact with the SWIRM domains of Swi3A and Swi3B by
electrostatic interaction within the nucleosome-binding module.
"interact with the SWIRM domains of Swi3A and Swi3B by electrostatic interaction"
Swi/Snf chromatin remodeling regulates transcriptional interference and gene repression
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Swi/Snf chromatin remodeling is required for co-transcriptional nucleosome
remodeling that drives LUTI-based gene repression via transcriptional interference.
"playing a direct role in gene repression via a cis transcriptional interference"
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The swi3-E815X allele (a nonsense mutant of the structural subunit Swi3)
reduces Swi/Snf repressive activity at proximal TSSs, though less strongly
than the snf2-W935R ATPase mutant.
"swi3-E815X mutation also reduces repressive activity by Swi/Snf at the TSSPROX but to a lesser extent than the snf2-W935R mutant."