This guide separates source identity, actual assay scope and access. Each publication cache is unchanged. The full SHA-256 hashes and assessed section locators are recorded in original-locators.json. No publication quotation is asserted.
Original source. Original abstract plus the literal bibliographic reference 20 in PMID:10900014; full Methods and Results unavailable.
Identity is authenticated as reference 20 of PMID:10900014 and independently matched to primary metadata. The accessible abstract reports positive RNA competition in rabbit reticulocyte lysates and binding capacity under the designation eIF-2/2B. Full Methods and Results were not recovered: factor composition/species, direct contacting entity and gamma dependence remain unresolved. The designation does not establish a mixed preparation, and the capacity comparison is not recast as an affinity constant.
Normal cache publications/PMID_1647211.md: full_text_available: false.
Original source. Complete normal abstract assessed; original Methods and Results were unavailable.
The exact rat P70541 donor record and primary abstract report tissue-dependent whole-eIF2B changes in diabetic rats and biochemical regulatory context. This supports a conserved contextual response without establishing a direct human gamma hormone receptor or sensor.
Normal cache publications/PMID_8430778.md: full_text_available: false.
Original source. Complete normal abstract assessed; original Methods and Results were unavailable.
The exact rat P70541 donor record and primary abstract support glucose-responsive whole-eIF2B activity in rat islets, including the reported nonmetabolizable analogue comparison. Complete Methods and a gamma-specific human mechanism remain unverified.
Normal cache publications/PMID_8567668.md: full_text_available: false.
Original source. Selected original Results, captions and Discussion concerning stimulated lymphocytes and whole-eIF2B activity, indexed blocks 17–38. Complete Experimental Procedures unavailable.
Selected original Results and captions support increased whole-eIF2B activity after human lymphocyte activation. Beta abundance and proposed epsilon/GSK3 regulation are distinct from a demonstrated gamma-specific T-cell-receptor signaling step. Complete Experimental Procedures were unavailable, so the precise gamma TCR annotation remains unresolved.
Normal cache publications/PMID_8626696.md: full_text_available: false.
Original source. Complete normal abstract assessed; original Methods and Results were unavailable.
The exact rat P70541 donor record and primary abstract support whole-eIF2B activity changes during heat stress in rat H35 cells. The original body was not reconstructed; no direct human gamma-specific thermal sensor is inferred.
Normal cache publications/PMID_9341116.md: full_text_available: false.
Original source. Methods on expression and validation ribozymes; Results on HeLa HCV-IRES translation; Discussion paragraph citing reference 20. Cached indexed blocks 16–30, 33–49 and 54 were assessed.
The complete cached main text establishes human HeLa EIF2B gamma-dependent HCV-IRES translation through independently targeted validation ribozymes. The separate EIF2 gamma target and ribozyme RNA cleavage are distinct experiments. Discussion cites earlier eIF-2/2B RNA binding, but neither the current perturbation nor the accessible earlier abstract resolves the gamma contribution to that binding function. The cloned 452-residue length alone does not authenticate every natural product.
Normal cache publications/PMID_10900014.md: full_text_available: true.
Original source. Original Purification of eIF2 and eIF2B Methods; Results on native complex purification and activity. Indexed original lines 468–558, 648–717, 848–1114 and selected Discussion 1689–2008.
Selected original Methods, Results and Discussion identify native human HeLa cytoplasmic eIF2B and whole-factor GDP dissociation activity. The isolated beta cross-linking and recombinant-beta experiments are not gamma assays. The unchanged normal cache is abstract-only; separately recovered original windows support the complex contribution without assigning isolated gamma catalysis.
Normal cache publications/PMID_11323413.md: full_text_available: false.
Original source. Complete normal abstract assessed; original Methods and Results were unavailable.
The bibliographic identity and clinical abstract are verified. The foregrounded severe EIF2B5 case does not establish misattribution of a gamma annotation, but operative evidence for the specific gamma heat-response assertion remains inaccessible and unresolved. No complete original Methods or Results were recovered.
Normal cache publications/PMID_12499492.md: full_text_available: false.
Original source. Original Methods for EBV-transformed cell exchange assays; Table 1, patient 823; Results and Discussion. Indexed Methods 336–509, Table 1 lines 571–696 and Results/Discussion 511–784.
Original Methods and Table 1 identify patient 823 with EIF2B3 H341Q/H341Q and reduced exchange activity, reported as 61 ± 6 percent of control. The assay uses human EBV-transformed lymphocyte extracts and rat-liver eIF2 substrate. It supports gamma contribution to the assembled exchange factor, not isolated gamma chemistry. The normal cache remains abstract-only.
Normal cache publications/PMID_15054402.md: full_text_available: false.
Original source. Original human-subunit cloning and assembly Methods; Results/Figure 1 and bounded Figure 4 context. Indexed primary blocks 25–62.
The selected original human cloning, Methods and Results/Figure 1 establish gamma–epsilon association and gamma-dependent assembly of larger eIF2B complexes in tagged-subunit experiments. Human EIF2B3 was cloned from HEK293 RNA. Weak association potentially mediated through endogenous subunits and the separate beta/epsilon mutant assays remain distinct; no purified binary affinity or natural-isoform equivalence is inferred.
Normal cache publications/PMID_15060152.md: full_text_available: true.
Original source. Original patient Methods and genotypes; selected Results, figure captions and Discussion. Indexed lines 415–439, 1374–1429, 1526–1545 and 1850–1894.
Selected original patient Methods, Results, captions and Discussion retain the positive oligodendrocyte phenotype, but the five examined genotypes comprise four EIF2B5 cases and one EIF2B2 case. The gamma-specific source attribution remains unresolved rather than treated as a global negative for gamma. The unchanged cache is abstract-only and complete operative access was not recovered.
Normal cache publications/PMID_15217090.md: full_text_available: false.
Original source. Complete normal abstract assessed; original Methods and Results were unavailable.
The verified abstract reports eIF2B supplementation of a HeLa cell-free system and enhanced initiation on capped, uncapped and EMCV-IRES templates. Operative purification Methods remain unavailable; the host extract does not establish the species or sequence of each added factor. The positive whole-factor initiation contribution is corroborated independently.
Normal cache publications/PMID_16289705.md: full_text_available: false.
Original source. Independent assessment of original whole-complex exchange and stress-regulation experiments; supplementary Table S1 was not inspected.
The independent source assessment supports whole-complex exchange and cellular stress-regulation experiments. Delta-subunit ISRIB-rescue evidence is not a gamma-specific ligand-binding assay. Supplementary Table S1 was not read, and the normal cache remains abstract-only; a complete construct inventory is not claimed.
Normal cache publications/PMID_25858979.md: full_text_available: false.
Original source. Primary Methods describing the human translation comparator and Results/Figure 2h, distinct from S. pombe structural work. Indexed entries 7–25, especially 12, 19 and 23.
The primary main text separates S. pombe expression, structure and gamma–epsilon subcomplex experiments from the human-factor translation comparator in Methods and Figure 2h/Results. The human comparator supports gamma-containing eIF2B contribution to initiation, not an isolated human gamma exchange reaction.
Normal cache publications/PMID_27023709.md: full_text_available: true.
Original source. Author original Methods for human subunit coexpression, purification and nucleotide exchange; selected Results. Column-order text lines 172–214, 220–305, 475–580, 816–878 and 1153–1195.
The author original includes human gamma coexpression in a reconstituted five-subunit eIF2B preparation and direct GDP-exchange assays. Human eIF2B was expressed in bacteria, whereas human substrate eIF2 was expressed in yeast. The ISRIB pocket belongs to the beta/delta interface, not gamma. Natural short products and supplementary construct sequences were not functionally authenticated.
Normal cache publications/PMID_29599213.md: full_text_available: true.
Original source. Article abstract only; official PDB 6K72 entity 3 and EMDB 9841 macro 3 independently inspected for canonical gamma sequence and expression-source identity.
The bibliographic identity and abstract are verified; original article Methods were not recovered. Independently inspected official PDB 6K72 entity 3, chains E/F, and EMDB 9841 macro 3 identify two copies of the exact human canonical 452-residue gamma sequence expressed in E. coli. Substrate eIF2 gamma is a separate entity. Deposited sequence identity does not prove equivalent function of the natural 412- or 401-residue products.
Normal cache publications/PMID_31048492.md: full_text_available: false.
The official three-product boundary remains Q9NR50-1/Q9NR50-2/Q9NR50-3, with lengths 452/412/401. PDB 6K72 entity 3 and EMDB 9841 macro 3 resolve the canonical gamma; eIF2 gamma is a different protein in the same deposited assembly. No short-product functional equivalence is assigned.
The portable evidence provides exact source URLs, hashes and a raw-derived replay for the family tree, alignment, literal IBDs, target rule conditions, donor records, Reactome entity paths and HPA location reliability. The current term and source relations remain separate from the biological assessment. In particular, the two probable UniProt relation queries preserve the original enables fields while requesting review of the complex-contribution interpretation.
The three unresolved annotations are retained explicitly. Incomplete subunit attribution or unavailable operative details are not negative evidence, and positive whole-factor observations remain visible. No new process annotation is inferred from a phenotype, and no additional core is added simply to mirror every contextual source assertion.
The heat-response annotation is a supported non-core gene-level claim through the rat P70541 donor and PMID:9341116. This corroboration supports retaining the parallel TAS claim while leaving the exact gamma-specific content of PMID:12499492 unresolved. The latter paper's incomplete access is recorded as a source limitation, not a negative heat-response conclusion.
RNA-dependent translation-factor activity remains a distinct unresolved claim from the accepted initiation-factor activity. PMID:10900014 is accessible and supports human EIF2B gamma-dependent HCV-IRES translation; Discussion reference 20 is the authenticated PMID:1647211. The earlier positive EMCV-IRES observation uses the combined designation eIF-2/2B, and its unavailable Methods do not resolve factor preparation or gamma contribution. No isolated-gamma RNA-contact requirement is imposed.
For the broad cytoplasmic row, HPA's EIF2B3 subcellular record independently supports cytosol, as does the target-resolved Reactome entity. The original HeLa purification remains evidence for cytoplasm. For CNS development, ARBA00026561's matched condition set contains two gamma-family FunFams; the patient 823 exchange assay in PMID:15054402 measures a biochemical defect in transformed lymphocytes, not a developmental endpoint. The developmental prediction is therefore over-annotated without disputing the disease association.
The existing locator inventory distinguishes partial normal caches from separately assessed originals. These direct public links make the load-bearing observations easier to inspect without adding quotations:
| Claim | Public original and operative locator | Access boundary |
|---|---|---|
| Gamma association with tagged epsilon and complex assembly | PMID:15060152 / PMC381664, human-subunit cloning and assembly Methods, Results/Figure 1; saved indexed blocks 25–62 | The normal cache contains abstract and Discussion, not the operative gamma passages. Selected original Methods/Results were separately assessed; no purified binary affinity is claimed. |
| Human translation comparator | PMID:27023709 / PMC4833825, Methods and Results/Figure 2h; saved entries 12, 19 and 23 | Human-factor comparator is distinct from the S. pombe structure and subcomplex work. |
| Gamma patient exchange defect | PMID:15054402 original, EBV-transformed-cell Methods and Table 1 patient 823 | Normal cache remains abstract-only; selected original Methods, Results and Table 1 were assessed separately. |
Exact cache/extract SHA-256 values and the original access scopes remain in original-locators.json. A source URL or cache flag does not itself imply that complete operative text was recovered. The three unresolved annotations are RNA-binding activity, oligodendrocyte development and T-cell-receptor signaling; none is relabeled as biologically false.
The official GO:0003743 history records removal of its is_a relation to GO:0008135 on 2025-02-12. Current is_a ancestors for both terms are captured in ontology-review.json. Both retain GO:0180051 as an ancestor; accepting initiation-factor activity therefore does not automatically resolve the separate RNA-binding assertion. The contribution qualifier remains respected.
The Reactome links identify different levels of the same saved path: R-HSA-72670 is the reaction, R-HSA-72526 is its input complex, and R-HSA-72525 is the gamma component whose reference entity is Q9NR50. The saved source projections explicitly retain this input → hasComponent → referenceEntity chain and cytosol compartment. The component link in this guide and the reaction source in the review are therefore compatible rather than interchangeable identifiers.