Use of the ND evidence code for Gene Ontology (GO) terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

PMID:14562095

Global analysis of protein localization in budding yeast.

Genome-wide GFP-fusion localization assigned ECM30 to the cytoplasm; this is the primary experimental subcellular-localization evidence for the protein.

PMID:9335584

Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae.

The calcofluor-white cell-surface transposon-mutagenesis screen from which the ECM ("extracellular mutant") series, including ECM30, was named; source of the UniProt FUNCTION statement that ECM30 is required for correct cell-wall composition.

PMID:17330950

Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.

ECM30 is phosphorylated at Ser1065 (large-scale phosphoproteomics).

PMID:18407956

A multidimensional chromatography technology for in-depth phosphoproteome analysis.

ECM30 is phosphorylated at Ser635 (large-scale phosphoproteomics).

PMID:19779198

Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.

ECM30 phosphosites Ser635 and Ser1065 detected in a Cdk1-substrate phosphoproteome, suggesting possible cell-cycle-associated phosphoregulation.

PMID:40899782

Golgi_traff phylogeny reveals ancient eukaryotic genes with recent surprises: replication and diversification of HID1 domain-containing protein unique to Schizosaccharomyces.

Fission-yeast Hid1/SPAC17A5.16 maintains Golgi architecture; the comparative study explicitly identifies divergent budding-yeast Ecm30. This is direct source-ortholog evidence and comparative target context, not a direct Ecm30 localization or knockout experiment.

"microscopy revealed that the SPAC17A5.16 mutant lacks a stacked Golgi apparatus"

PMID:20620961

A consensus of core protein complex compositions for Saccharomyces cerevisiae.

The primary publisher Results identify and validate an Ubp15-Ecm30 complex and examine its metabolic-expression effects. The local cache is abstract-only, so target-specific Results text is documented by publisher link in notes rather than represented as a cached quotation.

PMID:20093466

The genetic landscape of a cell.

The primary full article links Ecm30 to the Gap1-sorting module and reports deletion-dependent sorting/transport defects. The local cache is abstract-only; the target-specific primary passage was checked in the author-hosted paper.

file:yeast/ECM30/ECM30-uniprot.txt

UniProt record for yeast Ecm30

Ecm30 belongs to the HID1 family and has a curated cytoplasmic localization.

"Belongs to the hid-1 family."