Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Identification of VCP/p97, carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners using membrane-based human proteome arrays.
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CHIP polyubiquitinates caytaxin.
"CHIP e.g. was found to efficiently polyubiquitinate caytaxin in vitro, suggesting that it might influence caytaxin degradation in vivo."
Brain-specific BNIP-2-homology protein Caytaxin relocalises glutaminase to neurite terminals and reduces glutamate levels.
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Caytaxin binds KGA directly and in a brain complex.
"Co-immunoprecipitation with specific BNIP-H antibody confirmed that endogenous BNIP-H and KGA form a physiological complex in the brain, whereas binding studies showed that they interact with each other directly."
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Caytaxin relocalizes KGA to neurite terminals and lowers glutamate.
"It also reduced the steady-state levels of glutamate by inhibiting KGA enzyme activity."
Nerve growth factor stimulates interaction of Cayman ataxia protein BNIP-H/Caytaxin with peptidyl-prolyl isomerase Pin1 in differentiating neurons.
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NGF enhances caytaxin-PIN1 binding.
"We have characterized the interaction between BNIP-H and Pin1 in differentiating neurons and have further shown that NGF-stimulation of PC12 cells strongly enhanced their interaction via distinct binding motifs"
Cayman ataxia protein caytaxin is transported by kinesin along neurites through binding to kinesin light chains.
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KLC1 binds caytaxin's ELEWED motif.
"The tetratricopeptide-repeat region of KLC1 recognizes the ELEWED sequence (amino acids 115-120) of caytaxin."
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Caytaxin is in axons and dendrites.
"In cultured hippocampal neurons, we found that caytaxin is distributed in both axons and dendrites in punctate patterns, and it colocalizes with microtubules and KHC."
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Caytaxin knockdown redistributes mitochondria.
"A part of caytaxin, however, colocalizes with mitochondria and suppression of caytaxin expression by RNAi redistributes mitochondria away from the distal ends of neurites."
A reference map of the human binary protein interactome.
BNIP-H Recruits the Cholinergic Machinery to Neurite Terminals to Promote Acetylcholine Signaling and Neuritogenesis.
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Caytaxin links KLC1 to ATP citrate lyase and transports it to terminals.
"Here, we demonstrate that ataxia-related protein BNIP-H/Caytaxin links kinesin-1 (KLC1) to ATP citrate lyase (ACL), a key enzyme for ACh synthesis, and transports it toward neurite terminals."
Mutations in a novel gene encoding a CRAL-TRIO domain cause human Cayman ataxia and ataxia/dystonia in the jittery mouse.
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ATCAY mutations cause Cayman ataxia; caytaxin is neuron-restricted.
"The gene ATCAY or Atcay encodes a neuron-restricted protein called caytaxin."
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Caytaxin has a CRAL-TRIO motif.
"Caytaxin contains a CRAL-TRIO motif common to proteins that bind small lipophilic molecules."
Expression of Caytaxin protein in Cayman Ataxia mouse models correlates with phenotype severity.
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Human caytaxin rescues ataxic Atcay mutant mice.
"As the expression of wild type human Caytaxin in mutant sidewinder and jittery mice rescues the ataxic phenotype, Caytaxin's physiological function appears to be conserved between the human and mouse orthologs."
Insights into the regulation of CHIP E3 ligase-mediated ubiquitination of neuronal protein BNIP-H.
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CHIP ubiquitinates caytaxin on four lysines.
"Additionally, CHIP-mediated ubiquitination of BNIP-H is completely inhibited when BNIP-H bears arginine mutations at four key lysine residues."
Cayman ataxia-related protein is a presynapse-specific caspase-3 substrate.
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Caytaxin is a presynaptic caspase-3 substrate.
"Thus, we concluded that Caytaxin is a novel substrate of caspase-3 at the presynapse."
UniProt entry Q86WG3 (ATCAY_HUMAN)
Affinage mechanistic annotation for ATCAY (human)