Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cohesin cleavage by separase required for anaphase and cytokinesis in human cells.
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Cohesin cleavage by separase is essential for sister chromatid separation in human cells.
"cohesin cleavage by separase is essential for sister chromatid separation and for the completion of cytokinesis"
Caspase proteolysis of the cohesin component RAD21 promotes apoptosis.
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Apoptotic RAD21 cleavage at Asp279 is performed by caspases-3 and -7, not separase.
"preferentially cleaved at Asp(279) by caspases-3 and -7 in vitro"
Mechanism limiting centrosome duplication to once per cell cycle.
Cleavage of cohesin rings coordinates the separation of centrioles and chromatids.
Kendrin is a novel substrate for separase involved in the licensing of centriole duplication.
Regulation of human separase by securin binding and autocleavage.
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Securin blocks substrate access to the separase active site, and securin destruction permits autocatalytic cleavage of separase at one of three sites.
"allowing rapid autocatalytic cleavage of separase at one of three sites"
Processing, localization, and requirement of human separase for normal anaphase progression.
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Separase co-localises with gamma-tubulin at centrosomes in prometaphase and metaphase and the signal is lost at anaphase.
"revealed colocalization of separase and γ-tubulin in centrosomes during the early stages of mitosis"
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Antisense depletion of separase increases anaphases with lagging chromosomes more than four-fold.
"Antisense oligonucleotide-mediated depletion of separase results in a >4-fold increase in the mitotic cells with lagging chromosomes"
Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2.
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Activated human separase cleaves SCC1 at Arg172 and Arg450 in vitro.
"separase cleaves Scc1 at the same sites that are cleaved at anaphase onset in vivo, Arg172 and Arg450"
Systematic analysis of human protein complexes identifies chromosome segregation proteins.
PP2A delays APC/C-dependent degradation of separase-associated but not free securin.
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Separase cleaves centrosomal cohesin and pericentrin-B/kendrin to promote centriole disengagement.
"Separase also cleaves centrosomal cohesin and pericentrin-B (kendrin), which promotes centriole disengagement and licensing of subsequent centrosome duplication"
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Structural basis of human separase regulation by securin and CDK1-cyclin B1.
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Securin is an extended pseudosubstrate that blocks the catalytic and docking sites of separase.
"Consistent with previous studies, we find that securin acts as an extended pseudosubstrate that blocks substrate binding at the catalytic site and at nearby docking sites."
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SCC1 linker sequences between cleavage site and docking motifs are required for cleavage.
"Deletion of these linkers, or replacement with a random sequence, abolished Scc1 cleavage in vitro"
Autocleavage of ESPL1 (Separase)
PTTG1 (Securin) sequesters ESPL1 (Separase)
ESPL1 (Separase) cleaves centromeric cohesin
Separation of Sister Chromatids
UniProt entry Q14674 (ESPL1_HUMAN)
Deep research report for human ESPL1 (falcon provider)