FMO3 source guide

All 23 original QuickGO objects map bijectively to 23 genuine TSV rows and 23 stored annotations. No extension, partner, qualifier, evidence code or original-reference field was reconstructed or dropped. The three HuRI partner contexts remain distinct. The genuine seed has no natural alternative products.

Source access

PMID:10759686

Original record. Normal cache publications/PMID_10759686.md; full_text_available: false.

Normal cache is abstract-only; selected original PMC Methods, Results and Discussion were read separately. Recombinant human FMO3 microsomes produce albendazole sulfoxide above control by HPLC. Human-liver analysis favors a larger CYP contribution, chiefly CYP3A4; solubility limited the substrate range and kinetic estimates did not reach saturation. No sole-pathway or in-vivo fractional-contribution claim.

PMID:25910212

Original record. Normal cache publications/PMID_25910212.md; full_text_available: true.

Normal cache contains partial text despite its full-text flag. Selected original Results and Methods were read from the preserved genuine CHN1 fetch archive, with exact source provenance. Three positive source-specific IntAct records preserve human P31513/O43889-2, yeast host and variant-feature scope. Original supplementary target cells and complete insert sequences were not replayed; paper-level GPCA is not attributed to this pair.

PMID:31515488

Original record. Normal cache publications/PMID_31515488.md; full_text_available: true.

Selected actual full-text Methods and exact positive human P31513/O43889-2 IntAct record read. Human WT/mutant ORFs were tested in yeast with autoactivation controls and repeat-screen criteria. An unsuffixed O43889 record is distinct. No target-specific orthogonal PCA, physiological transcription role or processed-CREB3 interaction is inferred.

PMID:32156684

Original record. Normal cache publications/PMID_32156684.md; full_text_available: false.

Normal cache is abstract-only; selected UCL author-accepted full manuscript Methods, Results and Figure 3 legend were read separately. ROOT and independent peer also viewed the actual Figure 3 page. Human FMO3 baculosomes produce smaller amounts of taurine than FMO1 at 2.5 mM hypotaurine, measured by NMR peak integration with n=2 and half-range. FMO1-specific spectra, high-resolution product confirmation, kinetic parameters and Fmo1/2/4 mouse deletion are not FMO3 experiments. No separate supplement audit.

PMID:32296183

Original record. Normal cache publications/PMID_32296183.md; full_text_available: true.

Selected actual Results and Extended Data Figure 1 caption read. Human ORF yeast two-hybrid screening included pairwise retesting and sequence confirmation. Nine exact positive IntAct records cover the three original FMO3 partner contexts. Dataset-level MAPPIT/GPCA benchmarking is not target-specific orthogonal validation; no supplementary target-table, complete insert-sequence or endogenous mammalian assay replay is claimed.

PMID:9536088

Original record. Normal cache publications/PMID_9536088.md; full_text_available: false.

Identifier, title and authentic abstract verified. Full Methods and the exact N,N-dimethylaniline experiment remain unverified. The abstract reports human FMO3 variants and impaired N-oxygenation but does not identify every mapped substrate assay. Existing specific activity is retained contextually using the curated assertion and actual PAINT placement; GO and UniProt mappings share this source and are not independent experiments. Independent PMID:9776311 and PMID:17531949 support TMA activity.

PMID:9776311

Original record. Normal cache publications/PMID_9776311.md; full_text_available: false.

Authentic original abstract read: baculovirus-expressed human FMO3 and human adult-liver microsomes produce TMAO measured by radiochemical HPLC. Full Methods and exact construct boundaries were not recovered. The paper compares FMO paralogs, which are not natural alternative FMO3 products.

PMID:17531949

Original record. Normal cache publications/PMID_17531949.md; full_text_available: true.

Selected original full Methods and Results read. Human FMO3 has a C-terminal hexa-His tag and was expressed in insect cells; limited purification meant the kinetic experiments used membrane fractions. Radiometric HPLC distinguishes TMA from TMAO; FAD and immunoreactive protein were measured separately. The human structural explanation is a yeast-template homology model. Disease variants are not natural products.

PMID:29116146

Original record. Normal cache publications/PMID_29116146.md; full_text_available: true.

Selected original full Methods and Results read. Human WT and N61S FMO3 were expressed in E. coli and purified from membrane fractions. ITC directly measures NADP+ binding, while stopped-flow spectra follow flavin reduction and reoxygenation. TMA turnover is measured through substrate-dependent NADPH consumption; benzydamine and tamoxifen products use HPLC. Structural docking is computational; mutant defects are not assigned to normal FMO3.

Selected primary read locators

These locators identify the material actually read; they do not claim a complete figure or supplement audit. Normal-cache line numbers refer to the pinned bytes below.

No successful deep-research provider output was available for this session. Known current provider credential/quota failures were not retried; this work uses manual research and openly scoped original-source access, with no provider-named substitute file.

Original context projection

Indices are zero-based; the stored order is the genuine seeder order. The private proof retains each complete original object, including original WITH/FROM order.

Exact interaction records

All cited source records are positive human-protein interactions tested in yeast; their methods and features were inspected. These are curated original-assay records, not a fresh experimental replication.

• Raw 3, partner O43889-2: intact:EBI-12713718|imex:IM-25511-1392; intact:EBI-12727978|imex:IM-25511-2020; intact:EBI-12701228|imex:IM-25511-764.

• Raw 4, partner O43889-2: intact:EBI-22136996|imex:IM-27438-2134.

• Raw 5, partner Q8IUN9: intact:EBI-25134577|imex:IM-25472-59888; intact:EBI-23627786|imex:IM-25472-72623; intact:EBI-24635543|imex:IM-25472-144715.

• Raw 6, partner Q96HU1: intact:EBI-24920708|imex:IM-25472-15675; intact:EBI-23053048|imex:IM-25472-40563; intact:EBI-24428849|imex:IM-25472-126344.

• Raw 7, partner Q9NTJ5: intact:EBI-23444634|imex:IM-25472-62598; intact:EBI-24530640|imex:IM-25472-135457; intact:EBI-23219731|imex:IM-25472-49863.

Source records are accessible via the IntAct PSICQUIC service. Partner identity/class records: CREB3, CLEC10A, SGSM3, SACM1L. Their curated functions establish partner classes; those partner papers were not newly read. Official term definitions and is_a paths were checked through QuickGO for GO:0140297, GO:0005102, GO:0032794 and GO:0019902.

Phylogeny and location provenance

Private provenance receipt FMO3-paint-er-fh-20261029-v1/panther-treeinfo.json.receipt.json (SHA256 9d3a90a80043d4deb0cce7c13922891da605a07e9c93c06c262bfb1e489e6a84):

{
  "url": "https://pantherdb.org/services/oai/pantherdb/treeinfo?family=PTHR23023",
  "resolved_url": "https://pantherdb.org/services/oai/pantherdb/treeinfo?family=PTHR23023",
  "status": 200,
  "retrieved": "2026-10-29T01:21:12.436869+00:00",
  "bytes": 4174794,
  "sha256": "ac023ca3976b2c90055f6f1704bac9903175d450f061407a037748354d88364c",
  "content_type": "application/json;charset=UTF-8"
}

Private provenance receipt FMO3-paint-er-fh-20261029-v1/panther-familymsa.json.receipt.json (SHA256 dd29bd684a69451a294693d67f4898f73225bab2a74e7ea21322062dddf33bb5):

{
  "url": "https://www.pantherdb.org/services/oai/pantherdb/familymsa?family=PTHR23023",
  "resolved_url": "https://www.pantherdb.org/services/oai/pantherdb/familymsa?family=PTHR23023",
  "status": 200,
  "retrieved": "2026-10-29T01:21:10.267717+00:00",
  "bytes": 14886135,
  "sha256": "5dd17f69cd9d975b563a9f7a04344e6f6676b1e7585bb4c80a872344a80fcffb",
  "content_type": "application/json;charset=UTF-8"
}

Private provenance receipt FIG4-intake-20261028-v1/IBD.gaf.receipt.json (SHA256 354b701f91feb1ca87700a1d21691595132fdde86d1a324ef3921050435de448):

{
  "url": "https://data.pantherdb.org/ftp/downloads/paint/current/IBD.gaf",
  "status": 200,
  "bytes": 7600851,
  "sha256": "0c672914e10c1f79d3fb365077d843a92e518eb6eee2aac00c67150274adf569",
  "last_modified": "Fri, 28 Aug 2026 23:57:18 GMT",
  "fetched_at": "2026-10-28T05:21:02.332378+00:00"
}

The complete family tree/MSA has 1488 nodes and the exact 532-residue human target. The four-row normal PAINT slice was produced by the repository parse_ibd_gaf/write_family_paint helpers. Positive origins PTN000545689 and PTN000545693 are on the target path. IBD and tree/MSA releases are separately recorded.

Rabbit donor P32417 is FMO3 in Oryctolagus cuniculus. Human P31513 transfers the location from that record. ContentService event queries R-HSA-139970 and R-HSA-5602966 were followed through the exact entity IDs described in the evidence note, including all five disease-set members.

Immutable normal sources

Eleven sources were hydrated from exact main before fetch. Two additional publications were genuinely fetched, and the missing PAINT slice was genuinely derived from original data. All 14 are preserved unchanged after acquisition.

Review provenance

Accepted complete matrix: ac04b50ea0a1a564a895e64280147b49feb507b7b34b5d9901a38ee5ccc8806c. Genuine seed: b72ce236cf0dc20c8abbd9d38aa97d2846efdc42dc207475b4f2941bf46c0ac1. Full 23-context projection: c2100a3636c8c9d9168ca65bf0704edc804737cdd0d58c9614a0f09029e01532. Acquisition receipt: 025530686b03b7100fb0775d8553eff0162f6ef26bdca2311253e33d1846e306.

• Independent consultation receipt peer.json: 7e5fc1f8c1bcc1b18589fb76e24064785f14b6ac8422ef234fd5f15884d28ede.
• Independent consultation receipt peer.json: ef2ec855f82e63c6335e30ce584af714ba9c2f8f73df0b6be238f00622ddc8b3.
• Independent consultation receipt root-review.json: 80e05281a3e02f788c404b14e5a1bf3a8f537fa9a5807853f22c199ec14ada22.

The enzyme consultation read selected original Methods/Results from PMC2039921, PMC5676948 and PMC2014938 and the UCL accepted manuscript for PMID:32156684. The older two enzyme papers remain abstract-bounded. Interaction consultation used exact IntAct pairs and selected original human-ORF/yeast Methods; no target-specific orthogonal test or complete insert reconstruction was assumed. The detailed evidence note distinguishes these limits.

Published campaign instructions specify no new publication quotations and permit supported generic interactions to remain non-core when an informative subtype is not established. This review instead refines all five binding assertions because the exact records and partner classes support it.

Follow-up: receptor-binding scope and reaction hierarchy

The official GO:0005102 record, retrieved 2026-10-29, defines binding to a receptor and separately advises considering receptor agonist activity when appropriate. Its related receptor-associated-protein synonym and narrower receptor-ligand synonym do not make every receptor binder an agonist. The refinement identifies CLEC10A's verified signaling-receptor class; it does not assign CLEC10A function to FMO3.

The retained IntAct file has the exact human P31513/Q8IUN9 records at lines 4, 5 and 7: EBI-25134577 (prey pooling), EBI-23627786 (array), and EBI-24635543 (validated two hybrid), all in a yeast host and all explicitly positive. These are stages/records from one publication, not independent studies. Human hepatic/ER FMO3 and myeloid cell-surface/endolysosomal CLEC10A contexts leave native encounter and physiological significance unestablished. No shared ER encounter, endogenous co-expression, agonism, or pair-specific MAPPIT/GPCA result is inferred. The partner is CLEC10A, so the enzyme-binding alternative is not established by FMO3 itself being an enzyme.

Official is_a path checks from GO:0034899 and GO:0047822 to GO:0004499 returned no paths on 2026-10-29; their complete is_a ancestor lists also omit GO:0004499. The specific dimethylaniline definition therefore remains distinct from the TMA and hypotaurine definitions for this review. Shared EC usage and historical FMO synonyms do not establish an ontology ancestor relationship. This is not a claim that the activities are biochemically disjoint. The three dimethylaniline assertions remain non-core, with curator support retained and the uninspected exact assay honestly disclosed.

Only the taurine IBA reason retains the PAINT-placement sentence; it is removed from the three non-IBA taurine/hypotaurine reasons. A new question records the nicotine reaction already present in the unchanged reviewed P31513 record (RHEA:58720, catalytic-activity block at lines 308–315). The underlying nicotine primary paper was not newly acquired or reviewed, and no new annotation follows. The existing single catalytic-core description already states bound FAD and NADPH dependence; accepted cofactor-binding annotations do not require duplicate core entries.