Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
MucR, a novel membrane-associated regulator of alginate biosynthesis in Pseudomonas aeruginosa.
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MucR is in the cytoplasmic membrane with a cytosolic C-terminus.
"Translational reporter enzyme fusions with LacZ and PhoA suggested that MucR is located in the cytoplasmic membrane with a cytosolic C terminus."
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MucR activates alginate production via a localized c-di-GMP pool.
"MucR specifically regulates alginate biosynthesis by activation of alginate production through generation of a localized c-di-GMP pool in the vicinity of Alg44"
NO-induced biofilm dispersion in Pseudomonas aeruginosa is mediated by an MHYT domain-coupled phosphodiesterase.
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Recombinant MucR has both diguanylate cyclase and PDE activity in vitro.
"In contrast, MucR displayed both diguanylate cyclase and PDE activity in vitro, which seemed regulated in a growth-dependent manner in vivo."
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mucR mutants are impaired in dispersion in response to NO and glutamate.
"Inactivation of mucR impaired biofilm dispersion in response to NO and glutamate"
Dimerisation induced formation of the active site and the identification of three metal sites in EAL-phosphodiesterases.
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Crystal structures of the MucR EAL domain bound to c-di-GMP and Mg2+.
"We have studied the EAL signature motif-containing phosphodiesterase domains from the Pseudomonas aeruginosa proteins PA3825 (PA3825EAL) and PA1727 (MucREAL)."
UniProt entry Q9I310 (MUCR_PSEAE)