Use of the ND evidence code for Gene Ontology (GO) terms.

ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

High-throughput localization data suggesting nuclear, cell division site, and spindle pole body association

"global analysis of protein localization in the fission yeast Schizosaccharomyces pombe"

file:SCHPO/SPCC16C4.02c/SPCC16C4.02c-deep-research.md

Deep research report on SPCC16C4.02c

Orthologous to human neurochondrin (NCDN), contains DUF1941 domain
Interacts with Sfi1 (spindle pole body component), Mcp5/Num1 (cortical dynein anchor), and Ecl1 (chronological lifespan extender)
The OpenAI deep-research report inferred SPCC16C4.02c is non-essential for viability and likely functions as a scaffolding/regulatory protein. The scaffolding/regulatory inference is retained, but the non-essential claim is OUTDATED and contradicted by current PomBase, which reports deletion_viability = inviable for SPCC16C4.02c (asi1).
Conserved across eukaryotes but absent in S. cerevisiae

Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast.

A GOLEM GO-enrichment classification figure legend lists "SPCC16C4.02" among the nuclear Ino80 chromatin-remodeler set of doxorubicin (DOXO) resistance factors. This does NOT establish Ino80 membership for SPCC16C4.02c (asi1): the experimentally manipulated gene throughout the paper (deletion mutant construction, epistasis spot assays, Figure S4) is Spcc16c4.20c, which PomBase confirms is a separate real ORF (SPCC16C4.20c = hap2, an Ino80 complex HMG box protein), not a typo for SPCC16C4.02c. The "SPCC16C4.02" token in the GOLEM figure legend is most parsimoniously a single-digit transposition typo for SPCC16C4.20c/hap2. Tay 2013 therefore provides no reliable experimental evidence for SPCC16C4.02c, and the Ino80 grouping is contradicted by this protein's neurochondrin/ARM-repeat domains, NCDN orthology, and curated physical interactors (none of which are Ino80 subunits).

"chromatin remodeler Ino80 (Nht1, SPCC16C4.02, Iec1, Ies2, Iec3, Ies4, Ies6, Arp5,"
Epistasis (ten-fold serial dilution spot) assays among Ino80-set mutants showed no synthetic increase in doxorubicin hypersensitivity in double versus single mutants, interpreted as the subunits acting within the same complex/pathway. These experiments were performed on Spcc16c4.20c (hap2) and other Ino80 subunits, not on SPCC16C4.02c.

"The lack of synthetic hypersensitivity exhibited by the DMs on DOXO compared to the SMs suggests that the whole complexes may be important for DOXO response"

file:SCHPO/SPCC16C4.02c/SPCC16C4.02c-deep-research-falcon.md

Falcon deep research report on SPCC16C4.02c

Falcon's headline claim - that SPCC16C4.02c is a component of the S. pombe Ino80 chromatin-remodeling complex and behaves epistatically with other Ino80 subunits in doxorubicin-resistance assays (Tay et al. 2013) - is an artifact of gene-ID conflation and is NOT accepted. The Ino80 membership and the epistasis experiments in Tay 2013 concern Spcc16c4.20c, which PomBase confirms is a distinct real ORF (SPCC16C4.20c = hap2, an Ino80 complex HMG box protein), not SPCC16C4.02c (asi1). This claim is therefore not used to support any annotation for SPCC16C4.02c.

"The most direct experimental evidence available from peer‑reviewed research indicates that **SPCC16C4.02c is a named component of the S. pombe Ino80 chromatin‑remodeling complex** and that **mutants involving SPCC16C4.02c behave epistatically with other Ino80 subunits in assays of doxorubicin resistance**, consistent with a role in the same chromatin remodeling pathway/complex rather than an independent parallel pathway."
The UniProt/InterPro neurochondrin/ARM-type-fold domain assignments could not be validated against any retrieved paper discussing these domains for SPCC16C4.02c, so domain-based functional inference is database-derived context rather than literature-confirmed evidence.

"The user-supplied UniProt/InterPro domain assignments (ARM-type fold; Neurochondrin/PF05536/IPR008709) could not be independently validated from the retrieved literature set because no accessible paper here discusses these domains in connection with SPCC16C4.02c."
No retrieved evidence establishes a direct biochemical activity, substrate specificity, transport substrate, or definitive subcellular localization for SPCC16C4.02c; available evidence is genetic-network membership and phenotype assays, not molecular mechanism.

"The available evidence is genetic network membership and phenotype assays, not molecular mechanism."