atsA (PA0183) review notes

Literature search (2026-11-05)

No deep-research provider was available. PubMed MCP and Europe PMC search
endpoints failed during this session, so research used the cached GOA/UniProt
papers (PMID:7744061, PMID:9748219, PMID:19554727) plus PMID:11435113, fetched
with ai-gene-review fetch-pmid. All four are abstract-only in the cache.

Findings

Purified PAO1 arylsulfatase, sulfate-repressed, Km 105 microM for
4-nitrocatechol sulfate; atsA cloned by complementation PMID:7744061.
No signal peptide; presumed cytoplasmic PMID:7744061.
Cys51 is converted to formylglycine; the Ser mutant is inactive PMID:9748219.
1.3 A structure: formylglycine hydrate nucleophile, active-site Ca2+
PMID:11435113.
Promiscuous phosphate monoester hydrolysis, kcat 620-fold below sulfatase
PMID:19554727. PseudoCAP
annotated this paper to GO:0008081 phosphoric diester hydrolase activity,
which does not match the abstract (PSEUDOCAP WRONG_TERM defect, PROBABLE).

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	other seeds	IBD stands without this seed?	verdict	rationale
PTN000819263	GO:0004065 arylsulfatase activity	6	320 (50 taxa)	GO:0004065, IDA, PMID:7744061 (PseudoCAP; also UniProt IDA PMID:9748219)	human ARSA P15289, ARSL (formerly ARSE) P51690, ARSF P54793; mouse Arsg MGI:1921258; rat Arsg RGD:1310381	Yes (human ARSA alone is a classic arylsulfatase)	SOUND	Purified enzyme assayed with 4-nitrocatechol sulfate. Node term fits an arylsulfatase clade.

No GO_CENTRAL defect. The phosphodiester row does not seed a worklist IBD.