Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Ca(2+) signalling is not required for chemotaxis in Dictyostelium.
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Disruption of iplA produces null cells in which chemoattractant-stimulated Ca2+ entry and the cytosolic Ca2+ rise are abolished, while resting Ca2+ and cAMP chemotaxis remain normal.
"Ca(2+) entry in response to chemoattractants is abolished"
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Sequence homology places IplA in the InsP3/RyR receptor family of ligand-gated Ca2+ channels.
"InsP 3 /RyR receptor family of ligand-gated Ca 2+ channels"
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Direct biochemical evidence that IplA is an InsP3 receptor is still lacking.
"biochemical evidence that IplA is an InsP 3 receptor is still lacking"
Influx of extracellular Ca2+ is necessary for electrotaxis in Dictyostelium.
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iplA is responsible for the chemoattractant-induced cytosolic Ca2+ increase, but iplA-independent Ca2+ influx underlies electrotaxis, so iplA-null cells electrotax normally.
"Different roles of the iplA gene in chemoattractant-induced"
The inositol 1,4,5-trisphosphate receptor is required to signal autophagic cell death.
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iplA is the only gene encoding an IP3 receptor in Dictyostelium, and its inactivation prevents DIF-induced autophagic cell death.
"inactivation of the iplA gene, the only gene encoding an inositol"
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The IP3R is a ligand-gated channel governing Ca2+ efflux from ER stores to the cytosol.
"a ligand-gated channel governing Ca(2+) efflux from endoplasmic reticulum stores"
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The iplA mutation prevents cellulose shell synthesis around cells treated with DIF.
"The iplA − mutation prevented cellulose shell synthesis around cells subjected to DIF"
Arachidonic acid is a chemoattractant for Dictyostelium discoideum cells.
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In the iplA-null HM1049 strain, arachidonate chemotaxis is only slightly affected by EGTA, unlike wild type, implicating IplA in the Ca2+-dependent arm of arachidonate chemotaxis.
"chemotaxis was only slightly affected by EGTA"
Mechano-chemical signaling maintains the rapid movement of Dictyostelium cells.
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Randomly moving cells show brief Ca2+ transients dependent on intracellular and extracellular calcium sources during mechanochemical signaling.
"small, brief, Ca2+ transients in randomly moving wild-type"
The IplA Ca2+ channel of Dictyostelium discoideum is necessary for chemotaxis mediated through Ca2+, but not through cAMP, and has a fundamental role in natural aggregation.
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iplA-null cells chemotax normally in cAMP gradients but lose the capacity to chemotax up spatial Ca2+ gradients.
"cells lose the capacity to undergo chemotaxis in response to a spatial gradient"
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IplA has a fundamental role in natural aggregation; null cells fail to reorient toward the aggregation center at the onset of each natural cAMP wave.
"has a fundamental role in natural aggregation"
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IP3 receptors are usually located in the ER and vesicle membranes and are also found at low levels in the plasma membrane.
"usually located in the membrane of endoplasmic reticulum and vesicles, but are also found at low levels in the plasma membrane"
Microtubule-Mediated Inositol Lipid Signaling Plays Critical Roles in Regulation of Blebbing.
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iplA (Ca2+ channel)-deficient cells extend blebs more frequently than wild type, implicating IplA in the regulation of blebbing.
"extended blebs more frequently than wild type cells"
An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.
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iplA-null cells fail to move away from the chemorepellent AprA, so IplA is necessary for AprA chemorepulsion though not for cAMP chemoattraction.
"IplA and PkcA, although not necessary for chemoattraction toward cAMP, are necessary for chemorepulsion from AprA"
An Autocrine Negative Feedback Loop Inhibits Dictyostelium discoideum Proliferation through Pathways Including IP3/Ca(2).
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Cells lacking IplA have reduced sensitivity to polyphosphate-induced proliferation inhibition, and polyphosphate-induced cytosolic Ca2+ upregulation requires IplA.
"inositol 1,4,5-trisphosphate (IP3) receptor-like protein A (IplA), polyphosphate"
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Polyphosphate upregulates cytosolic Ca2+ through a pathway that requires IplA among other components.
"Polyphosphate also upregulates cytosolic Ca2+, and this requires GrlD"
Calcium responses to external mechanical stimuli in the multicellular stage of Dictyostelium discoideum.
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IplA, the homolog of the IP3 receptor, is essential for the cAMP-induced cytosolic Ca2+ elevation, and Ca2+ bursts induced by mechanical stimuli in slugs are partially mediated by IplA (with an additional influx pathway).
"the calcium channel, IplA, the homolog of the IP3 receptor, is essential for its elevation"
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Mechanically induced Ca2+ bursts are partially mediated by IplA/ER release.
"response to mechanical stimuli are partially mediated by"
Intracellular Calcium Responses to External Calcium Stimuli in Dictyostelium.
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IplA is described as a Dictyostelium ligand-gated calcium channel analogous to the mammalian IP3 receptor, responsible for Ca2+ release from the ER.
"IplA, a Dictyostelium protein analogous to the mammalian IP3 receptor, is a ligand-gated calcium channel responsible for calcium release from the endoplasmic reticulum"
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A significantly smaller proportion of iplA-null cells respond to external calcium stimuli than wild type, implicating IplA in regulating intracellular calcium responses.
"only approximately 60% of iplA-null cells were responsive to the external calcium stimuli"
OpenScientist function-assignment analysis of the IplA IP3-gated channel hypothesis