SHR3 carries three IBA rows from GO_REF:0000033, all traced by GOA to
PANTHER:PTN002000498 in the PTHR28228 SHR3 family. The repository currently
has the PTHR28228 family and subfamily in interpro/panther/panther.obo, but no
cached PTHR28228 PAINT TSV or PTN record. I therefore recorded the PTN in each
propagation_review as SOURCE_STALE_OR_MISSING rather than inventing
source-node details that are not locally cached.
The GO:0005789 endoplasmic reticulum membrane and GO:0006888 endoplasmic
reticulum to Golgi vesicle-mediated transport IBAs are sound core functions for
Shr3. The original SHR3 paper established it as an integral ER-membrane protein
needed for amino acid permease export from the ER PMID:1423607, and the COPII
paper specifically described Shr3-mediated coatomer-cargo interactions and
packaging of amino acid permeases into ER-derived vesicles PMID:10564255.
The GO:0051082 unfolded protein binding IBA already had the right action,
MODIFY to GO:0044183 protein folding chaperone; I added structured
TERM_SCOPING_PROBLEM / GRANULARITY_MISMATCH metadata. Kota and Ljungdahl
showed that Shr3's membrane domain prevents aggregation of amino acid permeases
in the ER PMID:15623581. Myronidi et al. 2023 then used scanning mutagenesis
and split-ubiquitin substrate tests to show that Shr3 selectively engages
nascent amino acid permease segments in a cotranslational, transient way
PMID:37477900. Those papers support an activity term, not a generic
unfolded-protein-binding term.
I also reclassified the five GO:0005515 protein binding IPI rows from
MARK_AS_OVER_ANNOTATED to REMOVE, following the current review policy for
generic protein binding. The interactions are not treated as false; they are
just less informative than Shr3's direct client-specific chaperone function.
I searched 2023-2026 SHR3/YDL212W and yeast amino-acid-permease literature. The
only newer direct experimental SHR3 paper I found was Myronidi et al. 2023
PMID:37477900, which is now cached with full text. Later search results were
either database pages, theses, or contextual citations of the 2023 JCB work and
did not alter the GO action calls.