A copper-activated two-component system interacts with zinc and imipenem resistance in Pseudomonas aeruginosa.

CopR negatively regulates oprD and positively regulates the czcCBA operon.

"Altogether these results show that the CopR regulator, like CzcR, negatively regulates oprD and positively regulates the czcCBA operon"
CopR is a new negative regulator of the OprD porin and links zinc, copper and imipenem resistance.

"This study shows that CopR is a new negative regulator of OprD porin and that it links Zn, Cu, and imipenem resistances by interacting with the CzcRS TCS."
Direct binding of CopR to the cop box was not demonstrated; it is inferred from the Pseudomonas syringae CopR site.

"It is therefore very likely that the P. aeruginosa CopR regulator binds to this cop box"
CopR is 55% identical to the zinc-responsive regulator CzcR.

"CopR was found to display 73% similarity and 55% identity with the CzcR regulator, as determined by pairwise sequence alignment."

The Two-Component System CopRS Maintains Subfemtomolar Levels of Free Copper in the Periplasm of Pseudomonas aeruginosa Using a Phosphatase-Based Mechanism.

The CopRS regulon is induced by external copper and is constitutively active when CopS is deleted, consistent with CopS controlling the phosphorylation state of CopR.

"In contrast, the ΔcopS mutant strain showed a constitutive activation of all the genes of the CopRS regulon, even in the absence of the Cu2+ stimulus."
CopS is proposed to dephosphorylate CopR in the absence of copper, shutting down the transcriptional response.

"However, in the absence of Cu, CopS shuts down the transcriptional response to Cu+, likely dephosphorylating CopR."

file:PSEAE/copR/copR-notes.md

copR review notes

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods