Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Proteomic characterization of the human sperm nucleus.
-
The study generated a catalogue of proteins from highly purified human sperm nuclei.
"With this approach, 403 different proteins have been identified from the isolated sperm nuclei."
[Analysis of the human acidic epididymal glycoprotein-like molecule: isolation of cDNA and tissue localization].
-
Human CRISP1 is specifically expressed in epididymis and detected in reproductive-tract fluids and sperm.
"This antibody detected a major band of 30 kD and a minor band of 26 kD in the caput, corpus, and cauda regions of the epididymis, the ductus deferens, the sperm, and the seminal plasma."
-
Human CRISP1 localizes to epididymal lumina/epithelium and the postacrosomal sperm head.
"Immunohistochemical analysis showed that the human AEG-like molecule is located in the lumen and epithelium of distal ductus efferentes and epididymal ducts, and on the postacrosomal region of the sperm head."
Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).
-
Human CRISP1 is an epididymal secretory glycoprotein bound to the postacrosomal sperm head.
"Although this protein, designated ARP (AEG-related protein), is not the ortholog of rodent AEG, it resembles AEG in that it is an epididymal secretory glycoprotein that binds to the postacrosomal region of the sperm head."
Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg.
-
A tightly associated human CRISP1 population is present at the sperm surface.
"Sequential extraction of proteins from ejaculated human sperm revealed the existence of a population of ARP that is tightly associated with the sperm surface and thus, potentially capable of participating in gamete interaction."
-
Blocking human CRISP1 specifically inhibits a step after oolemma binding and leading to sperm-egg fusion.
"The antibody did not inhibit the occurrence of spontaneous or Ca(2+) ionophore-induced acrosome reaction, nor did it inhibit the ability of sperm to bind to the oolema, supporting a specific inhibition of the antibody at the sperm-egg fusion level."
Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3.
-
Blocking sperm CRISP1 or occupying its zona sites reduces human sperm binding to hemizonae.
"Results revealed that both anti-hCRISP1 and rec-hCRISP1 produced a significant inhibition in the number of sperm bound per HZ compared with the corresponding controls."
-
Human CRISP1 preferentially binds ZP3.
"Results revealed that rec-hCRISP1 mainly interacted with ZP3 in a dose-dependent and saturable manner, supporting the specificity of this interaction."
Mammalian cysteine-rich secretory proteins interact with plasma membrane Ca(2+) exporter PMCA4b.
-
Human CRISP1 interacts with PMCA4b through its N-terminal domain.
"Human CRISP1 (hCRISP1) and hCRISP3 also interacted with PMCA4b via the N-terminal domain."
-
Human CRISP1 delays PMCA4b-mediated calcium extrusion.
"Interestingly, hCRISP1 and rCRISP4 delayed PMCA4b-mediated calcium extrusion but hCRISP3 did not."
Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1).
-
Mouse Crisp1 loss impairs in-vitro zona penetration and fusion without abolishing natural fertility.
"In vitro fertilization assays showed that Crisp1(-/-) sperm also exhibited a significantly reduced ability to penetrate both ZP-intact and ZP-free eggs."
CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization.
-
Native rat CRISP1 regulates CatSper in mouse sperm and modulates hyperactivation/orientation.
"Moreover, patch clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca(2+) channel involved in hyperactivation and essential for fertility."
Impaired male fertility and abnormal epididymal epithelium differentiation in mice lacking CRISP1 and CRISP4.
-
Combined mouse Crisp1/Crisp4 loss reveals reproductive redundancy and epididymal phenotypes.
"Our data showed that the simultaneous lack of the two epididymal proteins results in clear fertility defects."
The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3.
-
An early study found epididymis-specific human CRISP1 but no significant sperm association.
"In contrast to findings on its rat counterpart epididymal protein DE/acidic epididymal glycoprotein (AEG), no significant association of CRISP-1 with human spermatozoa was observed."
PANTHER PAINT node-level annotations for PTHR10334
UniProt entry for human CRISP1 (P54107)
Manual literature synthesis for human CRISP1