The acquisition contained 478 raw GOA rows and 471 seeded annotation contexts. Seven duplicate-context collapses are documented in the acquisition reconciliation; no imported source object, qualifier, partner, taxon or product was rewritten during review. The official displayed human Q09472 product is 2,414 residues; laboratory constructs were not turned into natural products.
Eight disjoint manual lanes covered all 471 contexts. Whole-gene integration resolves general functions only with positive independent evidence and retains unresolved partner-specific assays. The public source guide supplies DOI links, actual read scopes and experimental boundaries. Supported generic binding is retained as non-core where no defensible specific functional term was established, following the ClinGen campaign instruction; demonstrated partner classes receive specific terms. No new publication quotations or NEW annotations were introduced.
The inherited PMID:23934153 title placeholder was replaced with the exact genuine normal-cache title. PMID:15919823 is UNVERIFIED: its read main text concerns STAT3 transport without a p300 acetylation assay, while its supplement was inaccessible. Independent evidence establishes the activity. No original citation was rewritten. PMID:18273021 supports engineered-p300 H4 assays through its inspected supplement; the main article remained unavailable. Linked correction notices for PMID:23870121,26302407 and PMID:34637754 retain their actual access limits.
The human HPA record supports nucleoplasm and an additional cytosolic pool. Reactome events R-HSA-9701531 and R-HSA-9760082 have cytosol event-level compartments, but their EP300 participant R-HSA-381325 is explicitly nucleoplasmic. Both source participant chains support the imported nuclear location. Two structured queries record the event/participant compartment inconsistency; no GOA gene-location correction is asserted. The source guide records the exact chains.
Initial annotation checkpoint counts: {"KEEP_AS_NON_CORE": 103, "ACCEPT": 217, "MODIFY": 115, "UNDECIDED": 36}. Two independently synthesized cores now describe acetyl-CoA-dependent protein-lysine acetylation and transcription coactivation, located in the nucleoplasm. The computed DRAFT status reflects validation advisories. At that initial checkpoint all annotation rows had been reviewed, with 36 explicitly unresolved. Remaining uncertainty includes damaged-DNA contact, pre-mRNA binding, mitochondrial organization, brown-fat regulation and specific interaction partners; missing access is not evidence of curator error.
The normal acquisition attempted Falcon (HTTP403) and then perplexity-lite (HTTP401/quota). Neither produced a research result, so no provider-named research file was fabricated. The manually assessed originals and database provenance are documented in the source guide.
Core catalytic evidence uses PMID:20019387 Dna2 acetylation and PMID:25818647 histone acetylation; human PolII coactivation is supported by PMID:18782771 and PMID:25519132. PMID:18644873 independently supplies PolIII recruitment/coactivation capability, including assays without added acetyl-CoA, and does not substantiate the PolII-specific process by itself. Alternative acyl donors and individual binding partners are not separate core units.
This review follows the ClinGen campaign evidence policy: no new publication quotations are added. Verified reference identifiers, explicit source-access limits and Methods/Figure locators carry the claim-level provenance in review reasons, reference assessments and the linked source guide. Reference-only citations do not claim a successful quotation-substring check.
Human PolII coactivation was established in the actual recovered originals, beyond the normal abstract cache. PMID:18782771 Figure 6E uses human HepG2 cells for p300-dependent reporter activation; its mouse MEF PolII ChIP in Figure 6B-D is a separate experiment. PMID:25519132 Figure 4 and Cell culture/RNA interference Methods use human 293-derived reporter cells and P300-specific RNAi. Mouse CNS profiling does not replace those human experiments. PMID:18644873 remains separate PolIII coactivation evidence supporting molecular-function capability, not the PolII-specific process by itself. The reference reviews and source guide now give the original access routes and precise locators. Core descriptions, terms and reference membership are unchanged.
All 36 previously unresolved source contexts were reassessed individually. Thirteen action changes were made: H3K122 acetyltransferase activity from PMID:23415232 is ACCEPT based on its explicit positive residue-specific abstract and the curated IDA assertion; twelve generic binding contexts with explicit named-partner or complex support become KEEP_AS_NON_CORE. Full-original access flags remain unchanged. The 23 remaining unresolved contexts have substantive attribution, partner, substrate/process or assay-scope gaps; missing access is not treated as contradiction. In particular, resolving BETA2-p300 does not resolve E47-p300, resolving SIRT2 association does not establish a tubulin assay, and a chromatin co-occupancy result is not automatically a physical binary interface. No new publication quotations, source assertions, products or references were introduced. The revised review has 218 ACCEPT, 115 MODIFY, 115 KEEP_AS_NON_CORE and 23 UNDECIDED.
Reference relevance is assessed against the gene's function: a paper directly establishing a named p300 interaction can be HIGH even when the partner-specific annotation is non-core. That label does not certify every mechanistic detail or imply that the complete original was accessible; those limits remain in each reference assessment. Unread donor PMIDs cited in propagation explanations identify provenance gaps, not independently verified supporting experiments.