Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.
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EGCG treatment abolishes expression of the prestalk marker ecmA (and prespore pspA), reflecting a block of Dictyostelium differentiation rather than a specific catechin-response function of EcmA.
"we detected almost no expression of prespore specific pspA and prestalk specific ecmA genes in EGCG treated cells at 10 to 12 h into starvation"
Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum.
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ecmA is a prestalk gene strongly upregulated during late development (16-18 h), coincident with culmination and fruiting-body formation.
"Expression of the prestalk genes ecmA, ecmB and ecmF ([19,36], could be detected as early as 12 h, but were up-regulated more dramatically between 16 h and 18 h"
Proteomic profiling of the extracellular matrix (slime sheath) of Dictyostelium discoideum.
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LC/MS/MS of purified slug extracellular matrix (slime sheath) directly identified EcmA as an expected structural ECM component.
"identified numerous expected (e.g. EcmA, EcmD, discoidin I, discoidin II)"
Prestalk-like positioning of de-differentiated cells in the social amoeba Dictyostelium discoideum.
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ecmA (via an ecmAO reporter) is used as the prestalk cell-type marker to track differentiation, sorting, and de-differentiation during development.
"Refed (RF) cells harbored prespore (pspA-GFP, green) and prestalk (ecmAO-RFP, magenta) markers."
Transcriptomic and metabolomic insights into light-mediated unicellular-to-multicellular transition in Dictyostelium discoideum.
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ecmA and ecmB encode extracellular matrix proteins expressed mainly in anterior prestalk cells and are transcriptionally upregulated by light.
"During multicellular development, the ecmA and ecmB genes encode extracellular matrix proteins, which are mainly expressed in the most anterior prestalk cells of slugs and can regulate the morphogenesis of Dictyostelium"
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Light exposure upregulates ecmA (and ecmB) transcript levels.
"Our findings revealed that both of ecmA and ecmB genes are upregulated when exposed to light"
Prestalk cell-differentiation and movement during the morphogenesis of Dictyostelium discoideum.