Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Pseudomonas aeruginosa relA contributes to virulence in Drosophila melanogaster.
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A PAO1 relA mutant makes no (p)ppGpp after serine hydroxamate; multicopy relA restores it.
"ppGpp and pppGpp were undetectable in the relA mutant, whereas wild-type PAO1 produced high levels of ppGpp and pppGpp"
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relA loss reduces Drosophila virulence and alters quorum-sensing signals, pyocyanin, elastase and RpoS levels (downstream phenotypes of the alarmone).
"Deletion of relA also resulted in reduced levels of the RpoS sigma factor."
The stringent response is essential for Pseudomonas aeruginosa virulence in the rat lung agar bead and Drosophila melanogaster feeding models of infection.
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The relA spoT double mutant is ppGpp-null under all starvation conditions tested; SpoT degrades ppGpp.
"no (p)ppGpp synthesis could be detected in the relA spoT double mutant under any growth condition tested, including starvation for nutrients such as amino acids, carbon, and iron"
SpoT-triggered stringent response controls usp gene expression in Pseudomonas aeruginosa.
The RelA/SpoT homolog (RSH) superfamily: distribution and functional evolution of ppGpp synthetases and hydrolases across the tree of life.
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Proteobacterial RelA arose by duplication of Rel and lost hydrolase activity; SpoT kept both activities.
"while RelA is clearly more divergent, consistent with its loss of hydrolase activity"