Gigaxonin recruits selected substrates to CUL3–RBX1 ubiquitin ligase complexes. Its substrate-adaptor role supports protein ubiquitination, proteasomal turnover and intermediate-filament organization. The review also retains evidence for MAP1B-dependent microtubule localization and stabilization. These findings do not establish autonomous ligase catalysis, motor activity or direct tubulin binding.
The review contains 32 original annotation contexts: 14 ACCEPT, five MODIFY and 13 REMOVE. Three cytoplasm claims are refined to cytosol, cytoskeleton location to microtubule cytoskeleton, and cytoskeleton organization to two supported processes. Generic protein-binding removals preserve the positive interaction evidence. No new annotation is proposed. The evidence file preserves the original records and source locators; the journal preserves the dated reasoning and changes.
Individual reference assessments distinguish selected reading from source availability. A full-text flag does not mean every image, section or supplement was inspected. For example, PMID:27000625 is now available in full, while the recorded reading remains its abstract and Figure 1–4 captions. Mouse DRG phenotypes are not direct human mitochondrial-transport activity.
The inspected GO paths are GO:0005829 part_of GO:0005737, GO:0015630 is_a GO:0005856, and both GO:0045104 is_a GO:0007010 and GO:0000226 is_a GO:0007010. The microtubule term includes associated proteins. The process refinements retain separate intermediate-filament and microtubule evidence, without narrowing further to spatial filament-meshwork organization from clearance data alone.
The single core retains substrate-adaptor activity. Its cellular facets are protein ubiquitination, proteasome-mediated ubiquitin-dependent protein catabolism and intermediate-filament organization. The first two are not ancestors of one another under the inspected is_a/part_of closure. No new annotation is manufactured by describing these supported core roles.
Five Reactome event-to-component chains reach human GAN entity 976014, reference Q9H2C0, in cytosol. Intermediate sets and relations are retained in the evidence file. Complex membership does not assign GAN the catalytic work of neddylation, deneddylation or CAND1 exchange.
The official human Q9H2C0 sequence has 597 residues and no declared alternative product. Partner suffixes identify partner isoforms, not GAN products. Engineered fragments remain distinct from the full-length sequence. PMID:19818708's selected original describes GAN 1–258, while the UniProt bibliography gives 1–254; the discrepancy is preserved.
The four incoming PAINT assertions use the PANTHER19 PTHR24412 book, distinct from the current PTHR45632:SF17 classification. The exact human sequence maps to AN1997/PTN002521105 below assertion node AN25/PTN001561260. Original IBD lines, donor sets and target path are retained, with no negative assertion found on that path. Target self-evidence is legitimate descendant grounding. The compact evidence contains the route and assertions; the complete family files are retained privately. Family-release reconciliation remains a provenance question in the journal.
Original BioPlex 2/3 GAN–NUDCD3 rows, the NDEx U2OS AP-MS edge and exact IntAct partner/method records are preserved with locators. These cellular associations are not purified affinity measurements. The recovered PMID:15778465 original contains the GAN Table I row; its percentages are sequence coverage, and repeated EGF columns are not new conditions. Selected Methods, opening Results, caption and footnote were read. PMID:25036637 has a partial extraction despite its full-text flag; additional selected prose and the IntAct LUMIER pair are available. Two purported spreadsheet downloads were HTML, so no workbook-cell verification is claimed. Haenig and PMID:36931259 retain abstract/curated-record limits.
The evidence table reconstructs all 32 original QuickGO records, including nulls, dates, qualifiers and donor order. Saved joins connect each record to the export and actual generated review row. All 16 export columns and the 597-residue sequence were reconciled. There are no NOT records or extension atoms, and no inherited GAN review or journal was present.
All 38 source files are preserved: 20 reused from main and 18 acquired publications/Reactome records. An initially projected but absent PTHR45632 PAINT filename was excluded rather than filled with an empty file. Six existing excerpts remain unchanged and match their sources. The append-only journal records earlier stages; the current amendment and its checks are documented in a new history record.