Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Chromosome loss from par mutants of Pseudomonas putida depends on growth medium and phase of growth.
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parA mutants give anucleate cells in M9 medium at the transition to stationary phase.
"However, in M9-minimal medium different parA and parB mutations gave between 5 and 10% anucleate cells during the transition from exponential phase to stationary phase."
The parAB gene products of Pseudomonas putida exhibit partition activity in both P. putida and Escherichia coli.
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Deleting parAB raises the frequency of anucleate cells, especially in deceleration phase.
"A parAB deletion mutant, compared with wild type, showed slightly higher frequencies of anucleate cells in exponentially growing cultures but much higher frequencies in deceleration phase."
Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB.
Soj (ParA) DNA binding is mediated by conserved arginines and is essential for plasmid segregation.
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The Soj DNA-binding arginines are conserved in P. putida ParA.
"The arginine residues are conserved among other chromosomal homologues, including the ParAs from Caulobacter crescentus, Pseudomonas aeruginosa, Pseudomonas putida"
UniProtKB P0A149 (Y002_PSEPK) flat file
Pairwise comparison of P. putida and P. aeruginosa ParA and ParB